Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of lamp primer and its kit for detecting the silkworm bipartite densovirus

A technology of densovirus and kit, which is applied in the field of LAMP primers and kits for the detection of silkworm bipartite densovirus, can solve the problem of high sensitivity rate and achieve high sensitivity, short detection time, and safety effects on people and the environment

Inactive Publication Date: 2020-03-31
翁源县家宝蚕业有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The kit has strong specificity, high sensitivity rate, and is easy and quick to operate, which overcomes the problem that existing detection methods cannot be directly applied in production, and is suitable for the screening of silkworm BmBDV

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of lamp primer and its kit for detecting the silkworm bipartite densovirus
  • A kind of lamp primer and its kit for detecting the silkworm bipartite densovirus
  • A kind of lamp primer and its kit for detecting the silkworm bipartite densovirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] materials needed:

[0045] The samples infected with silkworm BmBDV virus were collected from the Institute of Life Sciences, Jiangsu University; the primers and probes used were synthesized by Shanghai Sangon Biotechnology Company; the large fragment of Bst DNA polymerase was purchased from New England Biolabs Company; dNTP, purchased from Promega Company; betaine , magnesium sulfate, etc. were purchased from sigma; nucleic acid dye SYBR Green I.

[0046] Bombyx mori bipartite densovirus (BmBDV), Bombyx mori densovirus (BmDNV), Bombyx mori nuclear polyhedrosis virus (BmNPV), Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) and Bombyx mori microsporidia (N. b.) originated from Jiangsu University Institute of Life Sciences.

[0047] S1. Extraction of DNA from silkworm BmBDV samples:

[0048] (1) Take 20-30 mg of silkworm whole silkworm or silkworm excrement, or 20 µL of silkworm hemolymph, add 500 µL of lysate RA and grind the homogenate with a grinding rod, then add...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to LAMP primers and a kit for detecting BmBDV (bombyx mori bidensovirus), belonging to the technical field of virus and epidemic disease diagnosis. The primers are designed according to the conserved domains of the VP sequence of the BmBDV (bombyx mori bidensovirus) disclosed by GenBank; after DNA of the BmBDV (bombyx mori bidensovirus) is extracted, LAMP reaction adopting the primers is carried out on the DNA and the reaction liquid, after the reaction is finished, the result is judged according to the color change of nucleic acid dye SYBR Green I, and the result shows that after the reaction is carried out for 30min at 62 DEG C, the DNA of the BmBDV (bombyx mori bidensovirus) obtains efficient specific amplification. Compared with the conventional detection technology, the detection technology provided by the invention has the features of simplicity, convenience and rapidness, good specificity and high sensitivity, can be used by technical staff of the first production line and silkworm raisers on site, and are beneficial for the diagnosis and prevention of the viral disease.

Description

technical field [0001] The invention relates to a LAMP primer and a kit for detecting the BmBDV virus of the silkworm, in particular to a set of loop-mediated isothermal amplification (Loop-mediated isothermal amplification, LAMP) technology for detecting the BmBDV virus primer of the silkworm and a kit thereof, belonging to Viral disease diagnosis technology field. Background technique [0002] Bombyx mori Bidensovirus (BmBDV) belongs to the Densoviridae subfamily of the Parvoviridae family. BmBDV mainly infects silkworms, and the symptoms of silkworm larvae infected by the virus include anorexia, diarrhea and softening, emaciation, hypnosis, translucent chest and other phenomena, and finally stop eating. The virus has a high fatality rate, and the infection lasts for a long time, causing chronic silkworm disease, which is one of the important reasons for the production reduction of economic products such as silkworm cocoons in summer and autumn in my country. [0003] St...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/6844C12N15/11C12R1/93
CPCC12Q1/6844C12Q1/701C12Q2531/119
Inventor 吕鹏陈克平潘晔林锋姚勤杨艳华朱菲菲马上上陈亮聂志超
Owner 翁源县家宝蚕业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com