Cucumber breeding method based on induction of polyploid and polyploid plant identification method

A technology of polyploidy and cucumber, applied in horticultural methods, botanical equipment and methods, biochemical equipment and methods, etc., to achieve the effect of improving economic benefits

Inactive Publication Date: 2017-07-07
HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although plant polyploid mutagenesis technology has been widely used, the existing cucumber germplasm resources cannot meet people's needs due to the strong market demand for cucumbers.

Method used

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  • Cucumber breeding method based on induction of polyploid and polyploid plant identification method
  • Cucumber breeding method based on induction of polyploid and polyploid plant identification method
  • Cucumber breeding method based on induction of polyploid and polyploid plant identification method

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Experimental program
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Effect test

Embodiment 1

[0060] 1 Materials and methods

[0061] 1.1 Test material

[0062] The seeds of three types of cucumbers (South China type 'TZT', North China type 'D19', and European greenhouse type 'NS3') provided by the Cucumber Research Group of Hebei Science and Technology Normal University were used as experimental materials.

[0063] 1.2 Polyploid induction

[0064] After the three types of cucumber seeds germinated at 30°C for 36 hours, they were sown in 50-hole trays and placed in a solar greenhouse for seedling cultivation. When the two cotyledons are fully unfolded, put the cotton ball on the growing point, use a rubber dropper to absorb the colchicine solution, drop it on the growing point cotton ball, and completely soak the cotton ball. Three colchicine concentrations (0.1wt%, 0.2wt%, 0.3wt%) were used to treat once respectively at 7:00-8:00 in the morning and 5:00-6:00 in the afternoon, and the treatment times were 3 times, 5 times and 7 times, a total of 3 × 3 treatments, 10...

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Abstract

The invention relates to the technical field of plant mutation breeding, in particular, to a cucumber breeding method based on induced polyploidy and a polyploid plant identification method, comprising: cultivating germinated cucumber seeds into seedlings, using colchicine The solution is used to induce the growth points of the seedlings; and then the diploid and mutant plants are identified by plant morphology, stomata, flow cytometry and cytology. The method can effectively breed new polyploid lines of cucumber suitable for production application, thereby improving its economic benefits.

Description

technical field [0001] The invention relates to the technical field of plant mutation breeding, in particular to a method for breeding cucumbers based on induced polyploidy and an identification method for polyploid plants. Background technique [0002] Because of its "hugeness", polyploidy can improve crop yield and quality, enhance disease resistance and stress resistance, and is widely used in production. Polyploid breeding has been widely used in food crops (Luo Xiaojin and He Haohua, 2011), fruit and vegetable crops (Chen Jinfeng et al., 2004; Ma Yue et al., 2015, Amaral et al., 2015), ornamental plants (Li Han et al., 2009, Li Doudou et al. , 2013, Chen et al., 2016) and medicinal plants (Wang Chaoliang et al., 2007, Wang Yuehua et al., 2010) breeding work, used to improve the agronomic traits of diploid parents. Using polyploids as intermediate materials for breeding to obtain triploids (Diao Weiping, 2009; Diao et al., 2009), and additional line materials have impor...

Claims

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Application Information

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IPC IPC(8): A01H1/08A01G7/00C12Q1/02C12Q1/68
CPCA01H1/08A01G7/00C12Q1/6895G01N33/5097
Inventor 郑金双孙成振宋晓飞李晓丽闫立英
Owner HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
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