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Tissue Culture Rapid Propagation Method of Clematis unifolia

A technique of clematis unifolia and tissue culture, applied in the biological field

Active Publication Date: 2018-09-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing wild resources of Clematis simplicifolia are difficult to meet the needs of the growing medical field. In order to protect wild clematis simplicifolia resources and meet the application of the pharmaceutical industry, it is necessary to establish a In vitro regeneration system, which is of great significance to enrich the theory of plant tissue culture and apply biotechnology to protect germplasm resources and meet clinical applications.

Method used

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  • Tissue Culture Rapid Propagation Method of Clematis unifolia
  • Tissue Culture Rapid Propagation Method of Clematis unifolia

Examples

Experimental program
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Effect test

Embodiment 1

[0119] Embodiment 1, a kind of tissue culture rapid propagation method of Clematis unifoliate, carries out following steps successively:

[0120] 1), the selection of seed explant:

[0121] Select the achenes of Clematis unifoliate, put them in water, float to remove the shriveled seeds, and then peel off the pericarp under a stereo microscope (the pericarp is covered with fluff, which may easily cause incomplete sterilization and pollution) to obtain seeds.

[0122] 2), seed disinfection:

[0123] Soak the seeds in distilled water for 10-15 minutes, put them on the ultra-clean table, soak them in 70% (volume %) alcohol for 20-30 seconds, wash them with sterile water, and then use 1% available chlorine sodium hypochlorite Solution (add 2-3 drops of detergent for every 100ml solution) to sterilize for 6-8 minutes, rinse with sterile water for 3-5 times to obtain sterilized seeds; set aside.

[0124] 3), breaking of seed dormancy (seed germination):

[0125] Transfer the seed...

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Abstract

The invention discloses a tissue culture and rapid propagation method of henry clematis. The method is characterized by taking seeds of henry clematis as explants and successively comprising the following steps: breaking dormancy of the seeds; carrying out propagation culture of terminal buds; cutting the obtained cluster buds into single buds, then repeatedly carrying out propagation culture, or transferring the cluster buds into a rooting medium for carrying out rooting culture inducing calli of the leaves, differentiating the calli, carrying out somatic embryogenesis, carrying out rooting culture; and transplanting. Through the method, tissue culture and rapid propagation of the henry clematis are successfully achieved; a large quantity of seedlings can be rapidly cultured; a practicable development project is provided for GAP planting of traditional Chinese medicine materials.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for rapid propagation of clematis unifolia through tissue culture. Background technique [0002] Plants are a natural treasure house of medicines, and humans have used medicinal plants to prevent and treat diseases for thousands of years. About 75% of the world's population uses plants as a source of medicine for treating diseases (Xing Jianmin, 2001). About 25% of today's prescription drugs come from medicinal plants (Qiu Deyou, 2000). Plants are one of the important sources of compounds. The quantity of secondary metabolites synthesized and accumulated by plants for extraction is regarded as economical and practical raw materials, which are widely used in cosmetics, food and other industries. Industrialization coupled with urbanization continues to increase the pressure on natural resources. Due to habitat destruction and predatory overharvesting, many traditional Chin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 姜维梅邱英雄
Owner ZHEJIANG UNIV