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Triterpene compounds in piptoporus betulinus and preparation method thereof

A compound, the technology of stripping fungus, applied in the field of natural product chemistry

Active Publication Date: 2017-07-07
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few domestic researches on the chemical components of Betula spp., and there are research reports on its biological activity. Yang Lixia and others have found that Betula can produce mannanase and xylanase with biological activity.

Method used

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  • Triterpene compounds in piptoporus betulinus and preparation method thereof
  • Triterpene compounds in piptoporus betulinus and preparation method thereof
  • Triterpene compounds in piptoporus betulinus and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] a. Take 3 kg of Betula exfoliate tube fungus, after pulverization, use 5 times the volume concentration of 100% methanol aqueous solution to extract once at room temperature, evaporate the extract to dryness, and obtain the medicinal extract;

[0027] b, dissolving and dispersing the extract obtained in step a with water, then extracting 3 times with petroleum ether, collecting the extract, evaporating the solvent in vacuo to obtain the extract extract; extracting the remaining water layer and extracting with ethyl acetate for 3 Second, collect the ethyl acetate extract, combine, and dry to obtain a light yellow solid powder ethyl acetate extract extract;

[0028] c. The ethyl acetate extract obtained in step b is separated by reverse column chromatography, and gradient elution is carried out with methanol aqueous solution with a volume ratio of 10%-100%, and the volume 80%-100% methanol aqueous solution is eluted Fractions were combined, the solvent was recovered, and ...

Embodiment 2

[0030] a. Take 3 kg of the medicinal material of Betula exfoliate tuberculosis, after pulverization, use 5 times the volume concentration of 95% ethanol aqueous solution to extract 3 times at room temperature, combine the filtrates, evaporate to dryness, and obtain the medicinal extract;

[0031] b. Dissolve and disperse the extract obtained in step a with water, then extract it with petroleum ether for 4 times, collect the extract, evaporate the solvent to dryness in vacuo, and obtain the extract extract; extract the remaining water layer with ethyl acetate 5 times, the ethyl acetate extract was collected, combined, and dried to obtain a light yellow solid powder ethyl acetate extract extract;

[0032]c. The extract extract obtained in step b is separated by reverse C18 silica gel column chromatography, gradient elution is carried out with methanol aqueous solution with a volume ratio of 10%-100%, and the fractions eluted with 80%-100% methanol aqueous solution , recover the ...

Embodiment 3

[0034] a. Take 3 kg of the medicinal material of Betula exfoliate tuberculosis, after pulverization, use 10 times the volume concentration of 100% methanol aqueous solution at a temperature of 70 ° C to carry out heating and reflux extraction, and evaporate the extract to dryness to obtain an extract;

[0035] b. Dissolve and disperse the extract obtained in step a with water, extract 5 times with petroleum ether, collect the extract, and evaporate the solvent to dryness in vacuo to obtain the extract extract; extract the remaining water layer with ethyl acetate for 4 times , collect the ethyl acetate extract, combine, and dry to obtain a light yellow solid powder ethyl acetate extract extract;

[0036] c, the ethyl acetate extract extract obtained in step b is separated by reverse column chromatography, gradient elution is carried out with methanol aqueous solution with a volume ratio of 10%-100%, and the fraction eluted with a volume of 80% methanol aqueous solution, The sol...

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Abstract

The invention relates to triterpene compounds in piptoporus betulinus and a preparation method thereof. Three new triterpene compounds are prepared by adopting the following steps: extracting a piptoporus betulinus medicinal material which serves as a raw material with a solvent, separating by three or four modes of normal phase or reverse phase silica gel column chromatography, or sephadex LH-20 column chromatography and semi-preparative high performance liquid chromatography and the like, and detecting and analyzing by adopting thin-layer chromatography and high performance liquid chromatography, and the structures of the three new triterpene are determined by spectral data analysis, wherein the compound 1 is 3,4-open loop-24-methyl lanostane-4(28),8,24(31)-triene-3,21-dicarboxylic acid, the compound 2 is 3,16-dicarbonyl-lanostane-7,9(11),24-triene-21-carboxylic acid, and the compound 3 is 16 alpha,25-dicarbonyl-3- carbonyl-lanostane-7,9(11),23-triene-21-carboxylic acid. The compounds are subjected to in-vitro cytotoxic activity detection, and experiment result indicates that the triterpene compounds separated from piptoporus betulinus have different levels of cytotoxic activities, and can be used for preparing anti-tumor medicines.

Description

technical field [0001] The present invention belongs to the field of natural product chemistry, specifically to the field of extraction, separation and preparation of effective components of natural products, and in particular to the extraction and separation of four new triterpenoids with pharmacological activity from the medicinal material of Betula spp. Background technique [0002] Piptoporus betulinus (Bull.: Fr.) Karst is a large fungus of the genus Piptoporus in the Polyporaceae family (Polyporaceae). , an annual. Mainly distributed in my country's Heilongjiang, Jilin, Liaoning, Inner Mongolia, Gansu, Sichuan, Yunnan, Guizhou, Xinjiang, Tibet, Henan, Shaanxi, Fujian, Anhui, Hunan and other regions. Fruiting bodies are medium to large, sessile or nearly sessile. The cap is nearly fleshy to leathery, oblate hemispherical, flat, often protruding near the base, (4-24)×(5-35)cm, 1.5-9cm thick, smooth surface, initially stained white-brown, later It is brown, with a thin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00C07C51/42C07C51/47C07C51/48C07C57/26
CPCC07C51/42C07C51/47C07C51/48C07C57/26C07J9/005
Inventor 袁涛再娜布·吐合达洪韩建欣
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI