Immune colloidal gold test strip for detecting thyroglobulin and preparation method thereof

A technology of thyroglobulin and colloidal gold test paper, which is applied in the field of immunoassay, can solve the problems that the Tg detection method is not suitable for on-site rapid detection, and achieve the effect of fast detection speed, broad application prospect and strong specificity

Inactive Publication Date: 2017-07-07
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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  • Abstract
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Problems solved by technology

[0006] In order to solve the problem that the existing Tg detection method is not suitable for on-site rapid detection, the present invention proposes an immune colloidal gold test strip for detecting thyroglobulin and its preparation method

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  • Immune colloidal gold test strip for detecting thyroglobulin and preparation method thereof
  • Immune colloidal gold test strip for detecting thyroglobulin and preparation method thereof
  • Immune colloidal gold test strip for detecting thyroglobulin and preparation method thereof

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0026] 1. Main material:

[0027] Human thyroglobulin full length protein (Tg freeze-dried powder ab96518), Anti-Thyroglobulin antibody (5F9) (anti-thyroglobulin antibody, ab1984), Anti-Thyroglobulin antibody (5E6) (anti-thyroglobulin antibody, ab1982): abcam company;

[0028] Goat anti-mouse IgG, colloidal gold test strip experimental consumables package: Shanghai Jinbiao Biotechnology Co., Ltd.;

[0029] BSA: Beijing Suolaibao Biotechnology Co., Ltd.;

[0030] Sodium citrate, chloroauric acid: Tianjin Yingda Rare Chemical Reagent Factory;

[0031] Clinical samples: 3 in total, 2 serum samples (Tg value 1541ng / ml, 20ng / ml), 1 cervical lymph node puncture eluate sample (Tg value 46ng / ml when diluted 1000 times).

[0032] 2. Preparation method:

[0033] (1) Preparation of colloidal gold: Add 99ml of ultrapure water into the flask, heat to boiling sta...

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Abstract

The invention discloses an immune colloidal gold test strip for detecting thyroglobulin and a preparation method thereof. The immune colloidal gold test strip includes a thyroglobulin detection gold test strip and preparation method thereof, wherein the immune colloidal gold strip, a gold labeled pad, a nitrocellulose membrane and a water absorption pad that are partly overlapped on a PVC base plate in order. The immune colloidal gold test strip is characterized in that: the gold labeled pad is coated with a colloidal gold labeled antithyroglobulin antibody, the nitrocellulose membrane is equipped with a separate detection line and quality control line, the detection line is coated with another antithyroglobulin antibody, the quality control line is coated with goat-anti-mouse IgG. The test strip provided by the invention has the advantages of simple preparation method, simple and convenient use, fast detection speed, high specificity, and qualitative and semi-quantitative detection of thyroglobulin, fully meets clinical needs, and has broad application prospects.

Description

technical field [0001] The invention relates to an immunoassay method, in particular to an immune colloidal gold test strip for detecting thyroglobulin and a preparation method thereof. Background technique [0002] Thyroglobulin (Tg) is a glycoprotein with a molecular weight of about 660,000 secreted by the thyroid follicular epithelium, and is one of the basic raw materials for the synthesis of thyroid hormones. Determination of blood Tg level and lymph node fine needle aspiration eluate Tg level (FNA-Tg) are widely used in the diagnosis, treatment and follow-up of differentiated thyroid cancer, especially papillary thyroid cancer. The elevated preoperative blood Tg level suggests that the postoperative blood Tg level can be used as a more sensitive tumor detection index. In addition, the postoperative serum Tg level, whether in the state of thyroid stimulating hormone suppression or non-thyroid stimulating hormone suppression, is helpful for the evaluation of the persist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/544
CPCG01N33/544G01N33/558
Inventor 何向辉王义增
Owner GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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