Kohlrabi Gene and Its Application in Breeding Purple Miniature Tomatoes

Abstract

The present invention discloses Brassica oleracea genes such as BoPAP2 and BoTT8, a co-expression vector and applications thereof, wherein the sequence of the Brassica oleracea gene BoPAP2 is represented by SEQ ID No.3, the sequence of the Brassica oleracea gene BoTT8 is represented by SEQ ID No.6, and after the co-expression vector of the Brassica oleracea gene BoPAP2 and the Brassica oleracea gene BoTT8 is transformed into a micro-Tom explant, a stable genetically modified strain can be obtained. According to the present invention, the phenotype of the transgenic tomato plant shows that a large amount of anthocyanin is accumulated in the leaves, the flowers, the roots and the fruits, and especially the fruit at the mature stage fruit is black and purple due to the synthesis and the accumulation f a large number of anthocyanins; and new micro tomato variety has good market promotion prospects and good economic value, and provides good reference and idea for the cultivation and the screening of new varieties of other ornamental plants.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to a kohlrabi gene and its application in cultivating purple miniature tomatoes. Background technique [0002] Tomatoes, which have antioxidant and cancer-preventing properties, are a popular vegetable worldwide because they are rich in active substances such as lycopene and beta-carotene. The tomato, native to South America, has been a globally important crop since about the 16th century. Tomatoes introduced to Europe from Latin America were first cultivated as ornamental plants. After long-term excavation and creation of tomato germplasm by breeders all over the world, the germplasm resources of tomato have been greatly enriched. As a tomato mutant, Micro-Tom retains the model plant characteristics of common tomato, and has a short plant and short life cycle, which is conducive to saving indoor space and requires less water, so it is more suitable for ornamental plants...

AI Technical Summary

Problems solved by technology

At present, the leaves and fruits of miniature tomatoes used for ornamental plant cultivation are relatively single in color, which limits the industrial development of miniature tomatoes as ornamental flowers.

Traditional breeding generally breeds new varieties through crossbreeding, but this method has a long cycle, high cost, unsatisfactory breeding effect and unpredictable goals

Images