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A method for continuously synthesizing geranyl butyrate with enzyme catalysis in circulating fluidized bed

A technology of geranyl butyrate and fluidized bed, which is applied in biochemical equipment and methods, methods for supporting/immobilizing microorganisms, biochemical instruments, etc., can solve problems such as unspecified experimental methods and no follow-up reports, and achieve reaction Effects of mild conditions, increased yield and efficiency, and high reaction efficiency

Active Publication Date: 2020-03-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are few reports on enzymatic synthesis of geranyl butyrate in China. In 1994, Xiao Bin reported the use of enzymatic synthesis of geranyl butyrate, but did not specify the experimental method and the enzyme used, and there was no follow-up report

Method used

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  • A method for continuously synthesizing geranyl butyrate with enzyme catalysis in circulating fluidized bed
  • A method for continuously synthesizing geranyl butyrate with enzyme catalysis in circulating fluidized bed
  • A method for continuously synthesizing geranyl butyrate with enzyme catalysis in circulating fluidized bed

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Effect test

Embodiment 1

[0047] Embodiment 1: circulating fluidized bed reactor

[0048] Circulating fluidized bed reactor of the present invention, such as figure 1 shown. Its main body consists of fluidized bed body (A), constant flow pump (B), liquid storage tank (C), feed tank (D), constant temperature water bath (E), water stop clamp (F), on-line detector (G ), wherein the fluidized bed body (A) has an interlayer, and a constant temperature water bath (E) supplies circulating water at a certain temperature to maintain a constant temperature in the bed. There are two pipes on the upper and lower sides respectively. The lower right side in the figure is the inlet of the fluidized medium, and the upper part is the outlet of the fluidized medium. They are connected by a constant flow pump with a large flow rate to form a circulation loop to maintain the fluidized state; the lower left side As the feed liquid inlet, a constant flow pump with a small flow rate is used to feed the bed from the feed ta...

Embodiment 2

[0050] Embodiment 2: continuous production process flow optimization

[0051] Geranyl butyrate was synthesized in a circulating fluidized bed reactor with butyric acid and geraniol as raw materials and lipase as a catalyst.

[0052] A lipase-catalyzed continuous synthesis method for synthesizing geranyl butyrate. The present invention studies the effects of substrate concentration, substrate molar ratio, lipase addition, bed expansion ratio, and feeding and discharging speed on the esterification rate.

[0053] Operation of the circulating fluidized bed reactor ( figure 1 ):

[0054] The volume of the reactor is 26mL, and a certain amount of lipase is placed in it. Firstly, there is water circulation in the jacket of the reactor and an external constant temperature water bath is connected to control the temperature of the reaction system. Then use a constant flow pump to feed the feed liquid with a certain molar ratio of acid to alcohol into the reactor and circulate it to ...

Embodiment 3

[0062] The volume of the reaction system is 26mL, the concentration of butyric acid in the raw material solution is 0.03mol / L, the concentration of geraniol is 0.03mol / L, the solvent is n-heptane, the amount of lipase added is 7U / mL, and the bed expansion ratio is 1.4. The temperature was 45°C, and after batch reaction for 4 hours, the raw material liquid entered the reactor at a flow rate of 0.10 mL / min, and reacted for 12 hours. The esterification rate of geranyl butyrate is shown in the following table:

[0063] The esterification rate of geranyl butyrate under different continuous reaction times of table 1

[0064]

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PUM

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Abstract

The invention discloses a method used for enzyme catalytic continuous synthesis of geranyl butyrate in a circulating fluidized bed, and belongs to the technical field of food biochemical engineering. According to the method, butyric acid and geraniol are taken as raw materials, and lipase is taken as a catalyst to synthesize geranyl butyrate. The method comprises following steps: intermittent reaction of the raw materials under catalytic action of the enzyme is carried out; when an intermittent reaction stable state is achieved, the raw materials are delivered into a reactor at a certain flow flux for continuous reaction. The continuous reaction time is 3 times of an initial startup time or longer, the esterification rate is 70% or higher, and no dehydrating agent is added in reaction process.

Description

technical field [0001] The invention relates to a method for continuously synthesizing geranyl butyrate by enzymatic catalysis in a circulating fluidized bed, and belongs to the technical field of food biochemical industry. Background technique [0002] Geranyl butyrate naturally exists in essential oils such as lavender oil, and is mainly used as a blending spice for flavors such as rose, acacia, geranium, lily of the valley, bay leaf, lavender, etc. It is also used in the deployment of food flavors, such as ice cream , chewing gum, pastry, candy, etc. Due to its limited natural sources, it mainly relies on industrial synthesis at present. The traditional synthesis method is chemical synthesis, and the mixture is obtained, and the cost of subsequent separation is high; and because of the use of concentrated sulfuric acid as a catalyst, there are disadvantages such as severe corrosion of equipment and the need for three-waste treatment. The present invention uses enzymatic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/62C12M1/40
CPCC12M21/18C12M25/20C12P7/62
Inventor 冯骉王璐缪铭唐静
Owner JIANGNAN UNIV
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