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Method for evaluating phagocytic capacity and fluorescence measurement method

An evaluation method, fluorescence measurement technology, applied in the direction of fluorescence/phosphorescence, measuring devices, biological testing, etc., can solve the problems of constant maintenance function decline, etc., and achieve the effect of improving transparency

Active Publication Date: 2017-07-14
HAMAMATSU PHOTONICS KK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] On the other hand, it is known that there is a decline in homeostasis maintenance function due to a decline in phagocytosis

Method used

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  • Method for evaluating phagocytic capacity and fluorescence measurement method
  • Method for evaluating phagocytic capacity and fluorescence measurement method
  • Method for evaluating phagocytic capacity and fluorescence measurement method

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Experimental program
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Embodiment

[0065] Hereinafter, the present invention will be described in more detail based on examples. However, the present invention is not limited to the following examples.

[0066] [Evaluation of phagocytic ability of phagocytic cells]

[0067] Phosphate-buffered saline (PBS) containing 0.2 w / v% of pH-sensitive fluorescent particles (Green E. coli, manufactured by Molecular Probes) was added to the microtube and incubated at 37°C. Next, 3 μL of human peripheral blood collected with a lancet was added, and mixed by inverting slowly up and down several times. Next, after standing still at 37°C for 2 minutes, place the microtube in a figure 1 Fluorescence is measured in the fluorescence measuring device with the optical system shown. Fluorescence was measured for about 10 seconds, and the average value of the measured values ​​(control fluorescence intensity) was calculated.

[0068] Next, the microtube was incubated at 37° C. for a predetermined time (60 minutes, 80 minutes, 120 ...

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Abstract

The present invention relates to a method for evaluating phagocytic capacity of phagocytes by using a sampling containing whole blood without complicated operations. The method includes the following steps: incorporating a fluorescent substance into phagocytes in a sample containing whole blood to obtain a measurement sample, standing the measurement sample for 2 minutes or more, measuring the fluorescence of the fluorescent substance, and evaluating the phagocytic capacity of phagocytes based on the intensity of the measured fluorescence, in which the measuring the fluorescence of the fluorescent substance includes irradiating an intermediate region of gravity direction of a placed measurement sample with an excitation light, and detecting the fluorescence generated from the fluorescent substance by the excitation light.

Description

technical field [0001] The invention relates to a phagocytosis evaluation method and a fluorescence measurement method. Background technique [0002] Phagocytosis (phagocytosis) acts not only on foreign substances such as bacteria, virus, dust but also on foreign substances (unnecessary substances) produced in the living body such as dead cells, oxidized low-density lipoprotein (LDL), denatured protein, and is Cellular endocytosis (intracellular uptake). Foreign body removal by phagocytes such as macrophages and neutrophils plays an important role in maintaining homeostasis (homeostasis). [0003] On the other hand, it is known that the homeostasis maintenance function decreases due to the decrease of phagocytic ability. For example, there have been reports on the delay of wound healing due to aging and the decrease in the phagocytic function of macrophages infiltrating into the wound (non-patent document 1); in the infection of pneumonia bacteria caused by aging , a repo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486G01N21/6408G01N2021/6439G01N33/49G01N2201/062G01N21/6428
Inventor 张燃稻川裕之杣源一郎数村公子土屋广司森下直计
Owner HAMAMATSU PHOTONICS KK