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Raman spectrum based online concentration detection method of L-arginine-alpha-oxoglutarate

A technology of Raman spectroscopy and ketoglutarate, which is applied in the detection field of L-arginine-α-ketoglutarate, can solve the problems of difficult to meet the needs of real-time process quality control, indistinguishability, long analysis time, etc. , to achieve real-time analysis, avoid interference, and analyze quickly

Active Publication Date: 2017-07-14
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the real-time analysis of the continuous production of AAKG, the reactant Arg, AKG and the product AAKG coexist in a dynamically changing chemical reaction equilibrium, and the existing high-performance liquid chromatography cannot distinguish whether Arg and AKG in the measured solution are reactants. It is also a dissociated component of AAKG; and high performance liquid chromatography requires a long analysis time of more than 30 minutes, which is difficult to meet the needs of real-time process quality control
There is no report on the AAKG real-time analysis method for the solution reaction system

Method used

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  • Raman spectrum based online concentration detection method of L-arginine-alpha-oxoglutarate
  • Raman spectrum based online concentration detection method of L-arginine-alpha-oxoglutarate
  • Raman spectrum based online concentration detection method of L-arginine-alpha-oxoglutarate

Examples

Experimental program
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Effect test

Embodiment 1

[0033] This example is used to verify the accuracy of Raman spectroscopy for determining the content of AAKG-1 in aqueous solution.

[0034] (1) Turn on the Mars laser, set the power of the light source to 1095mW, then connect the Ocean Optics QE6000Pro Raman spectrometer, set the integration time to 10s, measure the average number of times to 3 times, and select 200-2000cm -1 The wave number is used as the scanning range, and the detection wavelength of the Raman spectrometer is 785nm. Selecting the average times of 3 average measurements can reduce the measurement error, and the Raman spectrum intensity response value of 10s integration time is better, 2000cm -1 The wave number is the upper limit of the detection wave number of the instrument, and it is between 200 and 2000cm-1 The wavenumber range corresponds to most of the Raman characteristic groups.

[0035] (2) Measure the Raman spectrum of pure water under the preset Raman spectrum parameters, and save the measurement...

Embodiment 2

[0048] This example is used to verify the accuracy of Raman spectroscopy for determining the content of AAKG-2 in aqueous solution.

[0049] (1) Turn on the Mars laser, set the power of the light source to 1095mW, then connect the Ocean Optics QE6000Pro Raman spectrometer, set the integration time to 10s, measure the average number of times to 3 times, and select 200-2000cm -1 The wave number is used as the scanning range, and the detection wavelength of the Raman spectrometer is 785nm.

[0050] (2) Measure the Raman spectrum of pure water under the preset Raman spectrum parameters, and save the measurement result as the background.

[0051] (3) Prepare AAKG-2 solutions with mass fractions of 0.06, 0.12, 0.18, 0.24, 0.30, 0.36, 0.42, and 0.48 as the calibration set, set the magnetic stirrer speed to 250r / min, and the temperature of the circulating water bath to 35°C. Stir until AAKG-2 is completely dissolved, and measure the Raman spectrum of the AAKG-2 solution relative to t...

Embodiment 3

[0062] This example is used to verify the accuracy of Raman spectroscopy for determining the content of L-arginine-α-ketoglutarate (1:1) in an aqueous solution when α-ketoglutarate exists in the solution.

[0063] (1) Turn on the Mars laser, set the power of the light source to 1095mW, then connect the Ocean Optics QE6000Pro Raman spectrometer, set the integration time to 10s, measure the average number of times to 3 times, and select 200-2000cm -1 The wave number is used as the scanning range, and the detection wavelength of the Raman spectrometer is 785nm.

[0064] (2) Measure the Raman spectrum of pure water under the preset Raman spectrum parameters, and save the measurement result as the background.

[0065] (3) Weigh 8.0078g of AAKG-1 solid in a 50mL beaker, add 16g of water, and prepare an AAKG-1 solution with a mass fraction of 0.3335. Stir at constant temperature until the AAKG-1 is completely dissolved, and measure the Raman spectrum of the AAKG-1 solution relative ...

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Abstract

The invention discloses a Raman spectrum based online concentration detection method of an L-arginine-alpha-oxoglutarate solution. The method comprises the following steps: 1) preset parameters of a Raman spectrometer are set; 2) a Raman spectrum of a pure solvent is measured and the Raman spectrum is set as the measurement background; 3) the Raman spectrum of a sample to be tested is measured, and spectral data are preprocessed; 4) whether there is a Raman peak within the wave-number range of 990-1030 cm<-1> is judged; if there is no Raman peak, the liquid to be tested contains no L-arginine-alpha-oxoglutarate; if there is a Raman peak, whether there is a Raman peak within the wave-number range of 850-870 cm<-1> is judged; if there is no Raman peak within the wave-number range of 850-870 cm<-1>, the liquid to be tested contains L-arginine-alpha-oxoglutarate; and 5) the correlation between Raman peak response value and concentration is established respectively through an external standard method, and content of L-arginine-alpha-oxoglutarate in the solution is analyzed. The method of the invention has the following advantages: detection time is short; operation is simple and convenient; and real-time analysis of each component to be tested can be realized.

Description

technical field [0001] The present invention relates to a detection method of L-arginine-α-ketoglutarate, specifically adopting an online detection method based on Raman spectrum to measure the solution of L-arginine-α-ketoglutarate in real time concentration. Background technique [0002] L-Arginine-α-ketoglutaric acid (AAKG for short) is an amino acid health product widely used as a sports nutrition agent. AAKG can increase oxygen delivery in the body to a certain extent, improve muscle strength and endurance; AAKG can also directly and quickly provide nutrition to liver cells, which helps to maintain liver function and improve the quality of prognosis of patients with liver diseases. [0003] AAKG can be obtained by reacting L-arginine (Arg) and α-ketoglutarate (AKG) in a solution system. According to the molar ratio of the reactants Arg and AKG, AAKG exists in two forms: a monoarginine salt with a molar ratio of 1 (AAKG-1) and a double arginine salt with a molar ratio ...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/65G01N2021/655
Inventor 朱子飞江燕斌关国强
Owner SOUTH CHINA UNIV OF TECH
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