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Novel antibody ELISA kit for porcine epidemic diarrhea

A porcine epidemic diarrhea and detection kit technology, applied in the field of immune detection, can solve the problem of no PEDELISA antibody detection kit, etc., and achieve the effects of short detection time, good stability and long storage time

Inactive Publication Date: 2017-07-14
SOUTH CHINA AGRI UNIV
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Problems solved by technology

[0002] Porcine epidemic diarrhea (PED) is a pig disease that seriously endangers the pig industry in my country. Antibody detection is the key to prevention and control. However, there is no mature PED ELISA antibody detection kit at home and abroad.
[0003] Although there are Korean Anjie PED ELISA antibody detection kits on the market, they can only detect IgA in colostrum, and these kits are not registered in China. Therefore, there is no mature and commercialized PEDELISA antibody at home and abroad. Detection kit

Method used

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  • Novel antibody ELISA kit for porcine epidemic diarrhea
  • Novel antibody ELISA kit for porcine epidemic diarrhea
  • Novel antibody ELISA kit for porcine epidemic diarrhea

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Embodiment Construction

[0025] A new type of porcine epidemic diarrhea antibody ELISA detection kit, including the enzyme label plate coated with porcine epidemic diarrhea antigen, negative control serum, positive control serum, 20X concentrated washing solution, enzyme-labeled antigen, substrate buffer A, substrate Object solution B, stop solution. During the preparation of the ELISA plate coated with porcine epidemic diarrhea antigen, the original coating used was PEDV-S protein obtained through the baculovirus expression system, the coating solution used was sodium carbonate buffer, and the blocking solution used was The above coating solution containing 1% gelatin. The PEDV S protein was cloned into the baculovirus expression vector system, transfected into insect cells, and the foreign protein expressed in vitro was obtained, and the protein was used as a diagnostic antigen to establish an ELISA antibody detection method, and the prokaryotic expressed protein was used as a coating antigen. Comp...

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Abstract

The present invention discloses a novel antibody ELISA kit for porcine epidemic diarrhea, wherein the novel antibody ELISA kit comprises an enzyme label plate coated with porcine epidemic diarrhea antigen, a negative control serum, a positive control serum, a 20X concentrated lotion, an enzyme labeled antigen, a substrate buffer liquid A, a substrate liquid B, and a terminating liquid. According to the present invention, the PEDV-S protein obtained by the baculovirus expression system is used as the coating antigen, and is labeled with horseradish peroxidase through a sodium iodate method to obtain the enzyme labeled antigen; and the novel antibody ELISA kit developed based on the enzyme labeled antigen can detect the IgG in the pig serum and can further detect the IgA in the colostrum, is firstly created in domestic and foreign, has advantages of good specificity, good sensitivity, short detection time, long storage time and good stability, can simultaneously detect a large number of samples, and provides important practical significance for the solving of the detection of the porcine epidemic diarrhea virus antibody in the large-batch pig serum samples.

Description

technical field [0001] The invention relates to the technical field of immune detection, in particular to a novel antibody ELISA detection kit for porcine epidemic diarrhea. Background technique [0002] Porcine epidemic diarrhea (PED) is a pig disease that seriously endangers the pig industry in my country. Antibody detection is the key to prevention and control. However, there is no mature PED ELISA antibody detection kit at home and abroad. [0003] Although there are Korean Anjie PED ELISA antibody detection kits on the market, they can only detect IgA in colostrum, and these kits are not registered in China. Therefore, there is no mature and commercialized PEDELISA antibody at home and abroad. Detection kit. Contents of the invention [0004] The technical problem solved by the invention is based on the PED antigen expressed by baculovirus, and develops an ELISA antibody detection kit with high sensitivity, specificity and stability, which is used for rapid detection...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/535
CPCG01N33/56983G01N33/535G01N2333/005
Inventor 宋长绪杨金易曾道平王磊李段
Owner SOUTH CHINA AGRI UNIV
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