Microcolumn enrichment sample introduction method

Abstract

The invention belongs to the field of sample pretreatment, and concretely discloses a microcolumn enrichment sample introduction method, which comprises the steps of adopting a microextraction column with an extraction function as a sample introduction needle, enabling a sample to flow through the sample introduction needle, and during the period, extracting an analyte into an extracting medium in the sample introduction needle; then filling the sample introduction needle with an organic solvent, keeping for a certain time, and enabling the extracted analyte to dissolve in the organic solvent in the microextraction column so as to form a sample introductions solution; finally, sealing one end of the sample introduction needle, directly inserting the other end of the sample introduction needle into a gas chromatography sample introduction port, enabling the sample introduction solution to expand by heat so as to automatically release from the sample introduction needle to the sample introduction port, and finishing the sample introduction operation. The microcolumn enrichment sample introduction method provided by the invention integrates separation, enrichment and sample introduction into a whole, the dosage of the sample and the organic solution is less, and the enrichment efficiency is high; the necessary nitrogen blowing concentration operation in a solid-phase extraction method is omitted, so that the work efficiency of sample pretreatment can be greatly improved.

Description

technical field [0001] The invention belongs to the field of sample pretreatment, and in particular relates to a microcolumn enrichment sample injection method. Background technique [0002] The main purpose of sample pretreatment is to convert the target substance in the sample into a measurable form through the process of extraction, purification and concentration, and to remove the interfering substances in the sample matrix. Traditional sample pretreatment methods include Soxhlet extraction, liquid-liquid extraction (LLE), etc. However, these methods have disadvantages such as long processing time, cumbersome operation, and large consumption of organic solvents to varying degrees. In order to solve these problems, a series of new sample pretreatment methods appeared later, such as ultrasonic-assisted extraction (USAE), microwave-assisted extraction (MWAE), pressurized solvent extraction (PLE) and supercritical fluid extraction (SCFE), etc. Effectively improve the extrac...

AI Technical Summary

Problems solved by technology

The difference is that liquid phase microextraction (mLLE) uses a very small amount of organic solvent (hundreds of microliters) to extract hundreds of milliliters of samples once, and the operation steps are not much different from traditional LLE.

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