Proteins regulating the content of anthocyanins in blueberry fruit and their coding genes and applications
A technology of fruit and coding, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve problems such as ineffective effects
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Embodiment 1
[0072] Embodiment 1, the acquisition of transcription factor VcMYB5 and its coding base
[0073] The total RNA of the blueberry variety 'Sierra' was extracted and reverse-transcribed to obtain cDNA, and then PCR amplification was performed using primer F and primer R using the obtained cDNA as a template.
[0074] Primer F: 5'-ATGAATTTATCTGAGACCAG-3';
[0075] Primer R: 5'-TCAAATCAACAATGATTCGG-3'.
[0076] The amplified product is sequenced, and it can be seen that the obtained cDNA nucleotide sequence is sequence 2 in the sequence table, compared with the sequence in Genbank, it is determined that it belongs to the blueberry R2R3-MYB class transcription factor, and the gene shown in it is named VcMYB5, The encoded protein is named VcMYB5, and its amino acid sequence is sequence 1 in the sequence listing.
[0077] The above cDNA (Sequence 1) can also be obtained by artificial synthesis.
Embodiment 2
[0078] Example 2. Establishment of a gene transient VIGS silencing system in blueberry fruit and a functional study of the VcMYB5 gene
[0079] 1. Acquisition of recombinant expression vectors
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