Polygonum orientale fermented plant growth-promoting bacterium and application of bacterium in prevention and control of diseases and insect pests

A technology for Polygonum orientalis and Polygonum orientalis, applied in the directions of plant growth regulators, application, fermentation, etc., can solve the problems of economic difficulties in the research and development and production of plant-derived pesticides, affecting the stability of active substances, and unfavorable energy-saving environmental risks, etc. Good insecticidal, disease prevention and growth-promoting effects, beneficial to extraction, and the effects of inhibiting the growth of miscellaneous bacteria

Inactive Publication Date: 2017-07-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, botanical pesticides and antibacterial agents are mostly extracted with organic solvents or decocted, 1) it is not conducive to energy saving and has a greater environmental risk; 2) affects the stability of active substances an

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for preparing pesticides by fermenting Polygonum orientalis as raw material, comprising the following steps:

[0019] (1) Select 5g of fresh and good-quality Polygonum orientalis as the main raw material for fermentation.

[0020] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, add domestic garbage accounting for 5% of the total liquid volume and 0.5% The starchy substances are used as the fermentation stock solution for subsequent use.

[0021] (3) Prepare LB medium, activate the original preservation strain, and vibrate at 100r / min for about 48 hours to obtain the fermented bacterial liquid for subsequent use;

[0022] (4) According to the inoculation ratio of 4%, the commercially available Bacillus pumilus, Bacillus thuringiensis and Bacillus amyloliquefaciens H47 were respectively taken in equal proportions and inserted into the fermentation stoc...

Embodiment 2

[0025] A method for preparing pesticides by fermenting Polygonum orientalis as raw material, comprising the following steps:

[0026] (1) Select 5g of fresh and good-quality Polygonum orientalis as the main raw material for fermentation.

[0027] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, add 10% of the total liquid volume of domestic garbage and 1% The starchy substances are used as the fermentation stock solution for subsequent use.

[0028] (3) Prepare LB medium, activate the original preservation strain, and vibrate at 100r / min for about 48 hours to obtain the fermented bacterial liquid for subsequent use;

[0029] (4) According to the inoculation ratio of 5%, the commercially available Bacillus pumilus, Bacillus thuringiensis and Bacillus amyloliquefaciens H47 were respectively taken into the fermentation stock solution in equal proportions, cultured and f...

Embodiment 3

[0032] A method for preparing pesticides by fermenting Polygonum orientalis as raw material, comprising the following steps:

[0033] (1) Select 5g of fresh and good-quality Polygonum orientalis as the main raw material for fermentation.

[0034] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, add domestic garbage accounting for 15% of the total liquid volume and 1.5% The starchy substances are used as the fermentation stock solution for subsequent use.

[0035] (3) Prepare LB medium, activate the original preservation strain, and vibrate at 100r / min for about 48 hours to obtain the fermented bacterial liquid for subsequent use;

[0036] (4) According to the inoculation ratio of 5%, the commercially available Bacillus pumilus, Bacillus thuringiensis and Bacillus amyloliquefaciens H47 were respectively taken and inserted into the fermentation stock solution in equal ...

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Abstract

The invention provides a method for preparing a pesticide by fermenting polygonum orientale. The method is technologically characterized in that the polygonum orientale is used as a main raw material and is assisted by domestic garbage, starchy materials and water, commercially available bacillus pumilus, bacillus thuringiensis and starch hydrolyzing bacillus H47 are used as fermenting strains, pH is kept to be 5-8 according to the ratio of the polygonum orientale to the domestic garbage to the starchy materials to the purified water of (2-5): (1-2): 0.01: (3-7), the 2-5% strain inoculation quantity of a system, the fermentation temperature of 25-35 DEG C and the rotating speed is 100-200 rpm, and the fermentation time is 6-9 days. Complete fermentation is achieved, so that various enzymes, vitamins, amino acids and trace elements are fully released, and the efficacy and function of the pesticide are improved. In addition, the physiologically active substances of the plant pesticide are also not affected.

Description

technical field [0001] A method for preparing pesticides by microbial fermentation, which involves using commercially available Bacillus pumilus, Bacillus thuringiensis and Bacillus amyloliquefaciens H47 freeze-dried powder, and using Polygonum orientalis as raw material to ferment and prepare pesticides, which belongs to bioengineering and fermentation technology field. Background technique [0002] Polygonum orientalis is an annual herb of Polygonaceae. Its stem is erect, strong, 1-2m high, with many branches on the upper part, densely spread villous hairs. Leaves broadly ovate, broadly elliptic or ovate-lanceolate, 10-20cm long, 5-12cm wide, apex acuminate, base round or nearly heart-shaped, slightly decelerate, margin entire, densely ciliate, both sides Densely pubescent, densely pilose on leaf veins; petiole 2-10cm long, with spreading villous hairs; stipules tube-shaped, membranous, 1-2cm long, villous, long ciliate, usually along the Herbaceous, green wings at the ...

Claims

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Application Information

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IPC IPC(8): C12P1/04A01N65/30A01N63/00A01N63/02A01P7/00C12R1/07
CPCA01N63/00A01N65/00A01N65/30A01N63/10C12P1/04C12N1/205C12R2001/07A01N2300/00
Inventor 廖祥儒吴秋兰万兵蔡宇杰管政兵
Owner JIANGNAN UNIV
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