Primer and method for identifying animal fur based on PCR technology

A kind of animal and fur technology, applied in the direction of biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problem of low DNA content, etc., and achieve the effect of simple method, broad application prospect and good specificity

Inactive Publication Date: 2017-07-21
SHANGHAI INST OF QUALITY INSPECTION & TECHN RES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since animal fur has been tanned, bleached and dyed, the DNA content in the tanned fur is small, highly degraded, and contains a large amount of enzyme inhibitors. Technical Difficulties

Method used

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  • Primer and method for identifying animal fur based on PCR technology
  • Primer and method for identifying animal fur based on PCR technology
  • Primer and method for identifying animal fur based on PCR technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1 The specificity of the mink primer

[0069] Collected 9 tanned skins of sables and ferrets from domestic large-scale mink sable farms and fur trading markets, and 15 common animal furs that are easily confused. DNA was extracted by the improved CTAB method, and the mink primer was amplified by PCR to detect mink. are ingredients. See Table 2 for sample information.

[0070] Table 2. Sample information used to detect mink specificity

[0071]

[0072]

[0073] Depend on figure 1 It can be seen that the amplification of the positive, negative and blank controls was normal, the target bands were amplified by the mink primers in sable and ferret, and the mink, green root mink, weasel and aihu were all negative; the specificity of the primers was good.

[0074] According to the sequence comparison of the PCR amplification products, the mitochondrial ND2 gene identity between ferret and pine marten (forest mink) Martes martes reached 99%; the mitochondrial ...

Embodiment 2

[0076] Example 2 The Specificity of Ferret Primers

[0077] Collect 18 yellow wolf and aihu tanned skins from domestic large-scale fur animal farms and fur trading markets, as well as 15 common animal furs that are easy to be confused. The DNA is extracted by the improved CTAB method, and the PCR method is used to amplify the weasel primers to detect the weasel. Element. See Table 3 for sample information.

[0078] Table 3. Sample information used to detect ferret specificity

[0079]

[0080]

[0081] Depend on Figure 5 It can be seen that the amplification of the positive, negative and blank controls was normal, the target bands were amplified by the Weasel and Aihu primers, and the mink, Qinggen mink, sable and ferret were all negative; the specificity of the primers was good.

[0082] According to sequence comparison of the PCR amplification products, the mitochondrial ND4 gene identity of the weasel and weasel (Weasel) Mustela sibirica reached 99%; See Figure...

Embodiment 3

[0084] The specificity of embodiment 3 mink primer

[0085] Collected 14 imported and hybrid mink skins from domestic large-scale fur animal farms and fur trading markets, and 13 commonly confused animal furs. DNA was extracted by the improved CTAB method, mink primers were amplified by PCR, and mink components were detected. See Table 4 for sample information.

[0086] Table 4. Sample information used to test mink specificity

[0087]

[0088]

[0089] Depend on Figure 8 It can be seen that the amplification of the positive, negative and blank controls was normal. Among the mink primers, only the mink amplified the target band, and the yellow wolf, aihu, sable and sweeping ferret were all negative; the specificity of the primers was good.

[0090] According to the sequence comparison of the PCR amplification products, the consistency of the mitochondrial COI gene between mink and mink Neovison vison reached 100%. See Figure 9 .

[0091] Detection limit detection ...

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Abstract

The invention provides a method for identifying animal fur based on PCR, which belongs to the field of material identification of animal fur. It includes the following steps: extract enough DNA from tanned animal fur products, respectively use common mink (sable, pine marten, American mink, Japanese mink), ferret (weasel, weasel) and mink-specific primers to use PCR Technology amplification, to identify whether there are mink, weasel or mink components in animal fur, and the detection limit can reach 20pg. The invention has the advantages of accurate results, high sensitivity, strong specificity, stability and objectivity, and the like. The present invention can be used as a confirmation method for fur material identification by macroscopic observation method and microscope detection method, provides objective results when doubts arise in transactions, and can also provide technical support for market supervision, and has broad application prospects.

Description

technical field [0001] The invention relates to the field of material identification, in particular to a PCR (Polymerase Chain Reaction, PCR) technology-based primer and method for animal fur identification. Background technique [0002] Animal fur is leather with fur stripped from animals. It is generally used in the form of fur, mainly including mink, fox, raccoon, rex rabbit and yellow wolf. Mink fur is known as "the king of fur" and has a high commodity value. China is the world's largest fur animal farming country, and its fur production accounts for more than half of the world's total. my country's mink fur production has grown rapidly in recent years. According to the China Leather Association's report on the number of mink, fox and raccoon skins in China (2015), the report shows that in 2015, the number of mink skins in China was about 44.5 million, and the number of fox skins was 14.5 million. About 10,000 sheets. There are many kinds of mink, among which mink is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12N15/10
CPCC12Q1/6888C12N15/1003C12Q1/6806
Inventor 顾文佳段文锋韦娇君谭玉静胡雪莲张奕南石建新庞贝妮徐琼陆壹
Owner SHANGHAI INST OF QUALITY INSPECTION & TECHN RES
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