A rapid molecular identification method for plush products
A molecular identification and product technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as difficulty in promotion and inability to meet the requirements of the development of the plush industry, achieve trade disputes, high sensitivity, improve The effect of sensitivity
Active Publication Date: 2020-05-15
JIAXING UNIV
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Problems solved by technology
This identification method relies too much on the working experience of the inspectors, and for cashmere, modified sheep wool and alpaca fibers with very similar morphology and structure, this method gradually shows its limitations and can no longer meet the requirements of the development of the plush industry
Although the image recognition method, infrared spectroscopy, real-time quantitative PCR method, gene probe method, targeted proteome technology and biochip method developed in recent years have their own advantages, they are difficult to promote due to various reasons.
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Embodiment Construction
[0038] In the specific embodiment of the present invention, the percentages appearing are all percentages by mass unless otherwise specified.
[0039] 1. Materials, reagents and instruments
[0040] Standard reference materials: pure cashmere collected from Inner Mongolia, and fine wool and alpaca from Shanghai First Textile Co., Ltd. Generally, there is no commercially available reference fiber mixture, and the fiber blend is prepared by quantitatively mixing two or three different reference materials (cashmere / wool+alpaca), as shown in Table 1, and the percentages in Table 1 are mass percentages. These reference mixtures were analyzed by microscopic examination.
[0041] Reagent: Biomiga kit; Instrument: PCR amplification instrument (5331 eppendorf gene amplification Germany); Freeze dryer (6870SPEX SamplePrep USA)
[0042] Table 1
[0043] Numbering Cashmere ratio (%) Ratio of Alpaca (%) Wool ratio (%) 1 0.5 0.5 99 2 1 1 98 3 5 5 90...
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The invention discloses a rapid molecule identification method for plush products. The rapid molecule identification method comprises the following steps: extracting mitochondrion DNA (Deoxyribonucleic Acid) from a plush product; performing PCR (Polymerase Chain Reaction) amplification by using a cashmere specific primer, a wool specific primer and an alpaca fiber specific primer; performing agarose gel electrophoresis on amplification products, observing results, judging that the plush product comprises components of cashmere, wool and alpaca fiber if corresponding strips occur, and judging that the plush product comprises no corresponding components if corresponding strips do not occur. As specific primers of the specific cashmere, wool and alpaca fiber are adopted for PCR (polymerase chain reaction) amplification, and agarose gel electrophoresis is further implemented, components of cashmere, wool and alpaca fiber in the plush product can be accurately quantitatively identified.
Description
technical field [0001] The invention relates to the technical field of molecular identification of plush products, in particular to a rapid molecular identification method for plush products. Background technique [0002] my country is a big country in the production, processing and export of plush products, and the total processing volume of plush fibers ranks first in the world. As one of the important plush products, cashmere has uniform fineness, smooth, waxy, soft and delicate feel, strong tensile force and high elasticity, making its texture, feel, and warmth significantly superior to other plush products. Gold" is called. In order to achieve the purpose of reducing costs and increasing profits, some unscrupulous manufacturers often mix other fibers with relatively low value in the process of processing cashmere products, such as cashmere, modified sheep wool, stretched sheep wool and alpaca. Fake behavior not only damages the interests of consumers, but also affects ...
Claims
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IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/156
Inventor 刘晓侠尤忠毓王玉洁
Owner JIAXING UNIV