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High-efficiency astragalus polysaccharide extraction method

A technology of astragalus polysaccharides and extraction methods, which is applied in the field of extraction of high-efficiency astragalus polysaccharides, can solve the problems of effective component release hindrance, high equipment requirements for microwave extraction methods, and difficulty in implementation, and achieve increased leaching rate, good extraction effect, and high extraction rate. Improved effect

Inactive Publication Date: 2017-07-25
海门市协和农产品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The extraction of polysaccharides from Radix Astragali is mainly done by direct water extraction or water extraction after degreasing. The medicinal part of Radix Astragali is the root, in which there are more cellulose, hemicellulose and lignin, and the release of active ingredients has a greater effect during the extraction process. Obstructive effect; the microwave extraction method requires high equipment and is difficult to implement

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A method for extracting high-efficiency astragalus polysaccharides, comprising the following steps:

[0016] a. Soak Astragalus membranaceus in water, soak for 1 hour, grind into powder, and make Astragalus membranaceus powder;

[0017] b. Astragalus powder was hydrolyzed with cellulase for 0.5 hours, refluxed at 60°C for 3 hours, added ethanol with a volume concentration of 75% for extraction, separated to obtain an extract, and cooled to below room temperature;

[0018] c. Add sodium acetate aqueous solution to the above extract, heat to 50° C., keep warm for 20 minutes, and then filter to obtain the filtrate;

[0019] d. Add ethanol with a volume concentration of 95% to the filtrate to reflux to extract lipids, heat up to 80°C, and after 15 minutes, precipitates appear, and dry at 85°C to obtain astragalus polysaccharide.

[0020] The mass ratio of Radix Astragali and water is 1:50 in a step.

Embodiment 2

[0022] A method for extracting high-efficiency astragalus polysaccharides, comprising the following steps:

[0023] a. Soak Astragalus membranaceus in water for 1.5 hours, then grind it into powder to form Astragalus membranaceus powder;

[0024] b. Astragalus powder was hydrolyzed with cellulase for 0.7 hours, refluxed at 70°C for 4 hours, added ethanol with a volume concentration of 75% for extraction, separated to obtain an extract, and cooled to below room temperature;

[0025] c. Add sodium acetate aqueous solution to the above extract, heat to 75°C, keep warm for 45 minutes, and then filter to obtain the filtrate;

[0026] d. Add ethanol with a volume concentration of 95% to the filtrate to reflux to extract lipids, heat up to 90°C, and after 18 minutes, precipitates appear, and dry at 88°C to obtain astragalus polysaccharide.

[0027] The mass ratio of Radix Astragali and water is 1:75 in a step.

Embodiment 3

[0029] A method for extracting high-efficiency astragalus polysaccharides, comprising the following steps:

[0030] a. Soak Astragalus membranaceus in water, soak for 2 hours, grind it into powder, and make Astragalus membranaceus powder;

[0031] b. Astragalus powder was hydrolyzed with cellulase for 1 hour, refluxed at 85°C for 5 hours, added ethanol with a volume concentration of 75% for extraction, separated to obtain an extract, and cooled to below room temperature;

[0032] c. Add sodium acetate aqueous solution to the above extract, heat to 100° C., keep warm for 1 hour, and then filter to obtain the filtrate;

[0033] d. Add ethanol with a volume concentration of 95% to the filtrate to reflux to extract lipids, heat up to 100°C, and after 20 minutes, precipitates appear, and dry at 90°C to obtain astragalus polysaccharide.

[0034] The mass ratio of Radix Astragali and water is 1:100 in a step.

[0035] Astragalus powder uses cellulase to hydrolyze the cell surface s...

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Abstract

The invention discloses a high-efficiency astragalus polysaccharide extraction method. The method comprises soaking radix astragali in water for 1 to 2 hours, grinding the radix astragali to obtain radix astragali powder, carrying out enzymolysis on the radix astragali powder through cellulase for 0.5-1h, carrying out backflow at a temperature of 60-85 DEG C for 3-5h, adding ethanol having a volume concentration of 75% into the enzymolysis product, carrying out extraction, separating the extract, cooling the extract to the room temperature or less, adding an aqueous solution of sodium acetate into the extract, heating the mixture to 50-100 DEG C, carrying out thermal insulation for 20min to 1h, filtering the extract to obtain a filtrate, adding ethanol having a volume concentration of 95% into the filtrate, carrying out reflux extraction to remove esters, heating the extract to 80-100 DEG C, keeping the temperature for 15-20min so that precipitates are produced, and drying the precipitates at 85-90 DEG C to obtain astragalus polysaccharide. Cell surface structures of the radix astragali powder are decomposed by cellulase and a part of a sugar substance is dissolved out. After temperature increasing, leaching efficiency of hot water-soluble intracellular content is improved. Immersion is conducive to water extraction and esters are removed through ethanol extraction so that extraction effects are good and the extraction rate of polysaccharide is further improved.

Description

technical field [0001] The invention relates to a method for extracting plant polysaccharides, in particular to a method for extracting high-efficiency astragalus polysaccharides. Background technique [0002] The extraction of polysaccharides from Radix Astragali is mainly done by direct water extraction or water extraction after degreasing. The medicinal part of Radix Astragali is the root, in which there are more cellulose, hemicellulose and lignin, and the release of active ingredients has a greater effect during the extraction process. Obstructive effect; the microwave extraction method requires high equipment and is difficult to implement. Therefore, it is necessary to provide a new technical solution to solve the above problems. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide an extraction method of astragalus polysaccharide with simple process and high extraction efficiency. [0004] The technical schem...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/00
Inventor 俞瑶
Owner 海门市协和农产品有限公司
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