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New uses of prodigiosin, a microbial metabolite

A prodigiosin, Serratia marcescens technology, applied in the direction of chemicals for biological control, biocides, applications, etc.

Inactive Publication Date: 2019-03-15
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In general, PG is a fat-soluble pigment, easily soluble in methanol and acetone, insoluble in water, and slightly soluble in polar acidic and alkaline aqueous solutions. Prodigiosin has anti-tumor, immunosuppressive, antibacterial, Anti-malarial and other biological activities, but so far, there is no report about the microbial metabolite prodigiosin killing nematodes

Method used

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  • New uses of prodigiosin, a microbial metabolite
  • New uses of prodigiosin, a microbial metabolite
  • New uses of prodigiosin, a microbial metabolite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Serratia marcescens ( Serratia marcescens ) NJZT-1 was activated on LB solid medium, and the single colony activated on the solid medium was picked and inoculated into the seed medium for 24 hours at 28°C and 200r / min. Inoculated into LB liquid medium, and continued to cultivate at 28°C and 200r / min at constant temperature for 24h to obtain Serratia marcescens NJZT-1 culture solution.

[0025] The Serratia marcescens NJZT-1 culture solution was centrifuged at 9000rpm for 15min, and the supernatant was taken, which was used as the prodigiosin LB solution.

[0026] Add absolute ethanol to the centrifuged residue, shake and extract repeatedly, let stand for 10 minutes, then centrifuge at 9000rpm for 15 minutes, and take the supernatant, which is the untreated prodigiosin ethanol solution.

[0027] Put the untreated prodigiosin ethanol solution into a retort bottle, rotatively evaporate, collect the concentrate of the prodigiosin ethanol solution in the retort bottle, and...

Embodiment 2

[0033] Evenly inoculate a small amount of pine wood nematodes on the potato sucrose agar medium covered with Botrytis cinerea mycelia and culture at a constant temperature of 25° C. for 7-10 days, and the nematodes basically eat up the mycelia. Put a glass funnel (10-15 cm in diameter) on the funnel stand, connect a piece of rubber tube (about 10 cm) underneath, and install a water-stop clip on the rubber tube. Use the cross-hatch method to pick out the medium from the dish and put it on the filter paper, put them together in the funnel, add sterile water, and the sterile water should submerge the separation material. After 8-12 hours, place a clean centrifuge tube under the rubber tube, slowly open the water-stop clip, and take 10-15mL of water samples. Centrifuge the obtained water sample, discard the supernatant, add sterile water, centrifuge again, and repeat three to four times to obtain a relatively pure nematode suspension.

[0034] After fully mixing the obtained pine...

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Abstract

The invention discloses an application of microorganism metabolic product prodigiosin in killing pine wood nematode with poison. Compared with the prior art, according to the novel application of prodigiosin, different solvent is adopted to dissolve haematochrome, through observing the effect of killing pine wood nematode with poison of prodigiosin in the different solvent and the different concentrations, the fact that serratia marcescens metabolic product prodigiosin has a great efficiency of killing pine wood nematode with poison is confirmed, and the novel selectable methods are provided for biological prevention and control of nematode. The application provides a foundation for exploiting the biological activity of metabolic product prodigiosin of serratia marcescens preferably.

Description

technical field [0001] The invention belongs to the technical field of application of microbial secondary metabolites, and in particular relates to the new application of prodigiosin. Background technique [0002] Pine wilt disease, also known as pine wilt disease, is caused by the pine wood nematode ( Bursaphelenchus xylophilus ) caused by Pinus ( Pinus ) A disease that kills tree species. At present, the disease is prevalent in China, Japan, South Korea, Portugal and other countries, causing the death of a large number of pine trees and causing serious damage to the national economy and ecological environment. Because the disease is characterized by the strong pathogenicity of the pathogenic nematodes, the rapid death of pine hosts, and the rapid spread of the disease, it is difficult to prevent and control pine wood nematode disease. The Matsuura test showed that the use of fensulfothion, thionazin, morantel-tartrate, mesulfenos, carbosulfan, cyanophos, ethiodathion, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/36A01P5/00
CPCA01N43/36
Inventor 赵银娟吴小芹林司曦许径达
Owner NANJING FORESTRY UNIV