Extraction and separation method of achnatherum inebrians toxin

A separation method and a technology for the drunk horse grass are applied in the field of plant component extraction, which can solve the problems of high cost, complicated operation, low yield and the like, and achieve the effects of low cost, simple operation steps and high yield.

Inactive Publication Date: 2017-08-04
陕西金冠牧业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing methods for extracting Drunken Horse Herba toxin generally have the defects of low yield, complicated operation and high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for extracting and separating Drunken Horse Herba toxin, the steps are as follows:

[0021] 1) Take 1800 grams of Drunken Horse Grass dry powder, soak it in hydrochloric acid solution at room temperature for three times, each time for 24 hours, combine the leachate obtained three times, and filter to obtain the total leachate, wherein the mass concentration of the hydrochloric acid is 3%;

[0022] 2) Evaporating and concentrating the total leaching solution under reduced pressure at 60°C to obtain a concentrated solution with a relative density of 1.05 at 60°C;

[0023] 3) passing the concentrated solution through a cation exchange resin and washing with water until neutral;

[0024] 4) Alkalinizing the washed cation exchange resin and putting it on the column, eluting with ethanol solution, and recovering the solvent to obtain a crude extract, wherein the mass concentration of the ethanol solution is 70%;

[0025] 5) The crude extract is separated and purifie...

Embodiment 2

[0028] A method for extracting and separating Drunken Horse Herba toxin, the steps are as follows:

[0029] 1) Take 1900 grams of Drunken Horse Grass dry powder, soak it in hydrochloric acid solution at room temperature for three times, each time for 24 hours, combine the leachate obtained three times, and filter to obtain the total leachate, wherein the mass concentration of the hydrochloric acid is 3%;

[0030] 2) Evaporating and concentrating the total leaching solution under reduced pressure at 62°C to obtain a concentrated solution with a relative density of 1.10 at 60°C;

[0031] 3) passing the concentrated solution through a cation exchange resin and washing with water until neutral;

[0032] 4) Alkalinizing the washed cation exchange resin and putting it on the column, eluting with ethanol solution, and recovering the solvent to obtain a crude extract, wherein the mass concentration of the ethanol solution is 70%;

[0033] 5) The crude extract is separated and purifie...

Embodiment 3

[0036] A method for extracting and separating Drunken Horse Herba toxin, the steps are as follows:

[0037] 1) Take 2000 grams of Drunken Horse Grass dry powder, soak it in hydrochloric acid solution at room temperature for three times, each time for 24 hours, combine the leachate obtained three times, and filter to obtain the total leachate, wherein the mass concentration of the hydrochloric acid is 3%;

[0038] 2) Evaporating and concentrating the total leaching solution under reduced pressure at 65°C to obtain a concentrated solution with a relative density of 1.08 at 60°C;

[0039] 3) passing the concentrated solution through a cation exchange resin and washing with water until neutral;

[0040] 4) Alkalinizing the washed cation exchange resin and putting it on the column, eluting with ethanol solution, and recovering the solvent to obtain a crude extract, wherein the mass concentration of the ethanol solution is 70%;

[0041] 5) The crude extract is separated and purifie...

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Abstract

The invention discloses an extraction and separation method of achnatherum inebrians toxin. The method comprises the following steps: 1) soaking 1800-2200g of achnatherum inebrians dry powder in a hydrochloric acid solution at room temperature for three times, 24h per time, merging lixivia obtained at three times, and performing filtration to obtain a total lixivium; 2) evaporating and concentrating the total lixivium at a reduced pressure at 60-70 DEG C to obtain concentrated liquor; 3) washing the concentrated liquor through a cation exchange resin to neutral; 4) alkalizing the washed cation exchange resin to the column, eluting the same with an ethanol solution, and recovering a solvent to obtain coarse extract; 5) performing column chromatography on silica gel twice on the coarse extract, and separating and purifying the coarse extract to obtain alkaloid; and 6) performing silica gel G and Gf sheet chromatographic examination on the obtained alkaloid. The extraction and separation method of achnatherum inebrians toxin disclosed by the invention can extract achnatherum inebrians toxin in achnatherum inebrians, is high in yield and simple in operating step, and has important market value and social value.

Description

technical field [0001] The invention relates to the technical field of extracting plant components, in particular to a method for extracting and separating equestrian toxin. Background technique [0002] Drunken horse grass is a perennial herbaceous plant of the genus Achnatherum splendens in the grass family. It is a famous poisonous weed in northern my country, especially in the northwest grassland. Equus animals can cause poisoning symptoms such as rapid heart rate and staggering gait after eating, so it is named drunk horse grass. On the one hand, the research on the extraction and separation method of the toxin from Drunken Horse Grass is because the toxin itself has important medical value; Detoxification. Therefore, how to efficiently extract the equestrian toxin is a hotspot of people's research. Existing methods for extracting the toxin of eucalyptus generally have the defects of low yield, complicated operation and high cost. Contents of the invention [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C209/86C07C211/63
CPCC07C209/86
Inventor 党晓鹏
Owner 陕西金冠牧业有限公司
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