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Method for screening anaerobic bacterium traditional Chinese medicine inhibitor based on FeSiCo nano probe

A technology of nano-probes and anaerobic bacteria, which is applied in the direction of measuring devices, instruments, and nuclear magnetic resonance analysis, and can solve problems such as the decrease of nuclear magnetic resonance relaxation time

Inactive Publication Date: 2017-08-04
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As mentioned earlier, a small amount of FeSiCo nanomaterials will cause a significant decrease in the NMR relaxation time of water.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0019] To screen traditional Chinese medicine formulas that have inhibitory or therapeutic effects on acne bacilli (anaerobic bacteria).

[0020] 1) Nano-FeSiCo immunoprobe preparation:

[0021] FeSiCo nanoparticles can be purchased from the market, or can be synthesized by physical or chemical methods with reference to literature.

[0022] Silica-coated nano FeSiCo: Take 47.5g of sodium silicate, dissolve it in a beaker with deionized water, and adjust the pH value to 12-13 with hydrochloric acid. Take 5.0g of nano-FeSiCo and add it into the beaker, and stir mechanically (with a glass rod) for 5min. The mixture was sonicated for 30 min and stirred in due course. Raise the temperature to 85˚C, add hydrochloric acid dropwise to adjust the pH value to 6-7, and form a precipitate. While magnetically separating, wash the precipitate with deionized water for 3-4 times. Then, the precipitate was dispersed in 250 mL of methanol. The above process is repeated three times to ensur...

example 2

[0027] To screen traditional Chinese medicine formulas that have inhibitory or therapeutic effects on Clostridium difficile (anaerobic bacteria).

[0028] 1) Nano-FeSiCo immunoprobe preparation:

[0029] FeSiCo nanoparticles can be purchased from the market, or can be synthesized by physical and chemical methods with reference to literature.

[0030] Silica-coated nano FeSiCo: Take 47.5g of sodium silicate, dissolve it in a beaker with deionized water, and adjust the pH value to 12-13 with hydrochloric acid. Take 5.0g of nano-FeSiCo and add it into the beaker, and stir mechanically (with a glass rod) for 5min. The mixture was sonicated for 30 min and stirred in due course. Raise the temperature to 85˚C, add hydrochloric acid dropwise to adjust the pH value to 6-7, and form a precipitate. While magnetically separating, wash the precipitate with deionized water for 3-4 times. Then, the precipitate was dispersed in 250 mL of methanol. The above process is repeated three time...

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Abstract

The invention discloses a method for screening an anaerobic bacterium traditional Chinese medicine inhibitor based on a FeSiCo nano probe, and belongs to the technical field of traditional Chinese medicine development application. The method depends on a method for making a traditional Chinese medicine formula for preparing a FeSiCo nano probe and screening inhibiting anaerobic bacteria. According to the method, by virtue of the characteristic that a paramagnetic FeSiCo nano probe wrapped by an antibody is specifically combined with a target bacterium, whether a sample comprises a target bacterium or not can be detected by virtue of the influence of the paramagnetic property of FeSiCo to the relaxation time of magnetic resonance imaging. The paramagnetic FeSiCo nano probe has a linear relationship with the relaxation time of magnetic resonance imaging within a certain range, that is, the larger the content of nano FeSiCo is, the smaller the values of magnetic resonance imaging spinning-crystal lattice relaxation time and spinning-spinning relaxation time of the sample are, target bacteria can be quantitatively detected within a certain range, and thus the bacterium resistance effects of traditional Chinese medicine formulae can be indirectly evaporated. By adopting the method, formulae of traditional Chinese medicines with an inhibiting function on anaerobic bacteria can be screened, and the development speeds of traditional Chinese medicines can be increased.

Description

technical field [0001] The invention relates to a screening method of traditional Chinese medicine formula. Background technique [0002] Antibiotics can also cause damage to the human body while sterilizing bacteria, affecting liver, kidney function, and gastrointestinal tract reactions. Abuse of antibiotics may lead to the occurrence of drug resistance in some bacteria, and the treatment of infections will become very difficult. At present, there is almost no antimicrobial drug without drug resistance. It is of positive significance to develop traditional Chinese medicines and formulas with antibacterial effects. [0003] Many traditional Chinese medicines and prescription medicines have antibacterial and anti-inflammatory effects, and the active ingredients and molecular structures of some Chinese herbal medicines have also been fully or partially studied. For example, berberine (berberine), the main component of Coptis chinensis and Phellodendron phellodendron for dys...

Claims

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Application Information

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IPC IPC(8): G01N24/08
CPCG01N24/088
Inventor 张锦胜郑洪立巫小丹邹灯超彭红万益琴赵茹
Owner NANCHANG UNIV