Human body anti-AQP4 autoantibodies detection material and preparation method thereof
A technology for detecting materials and autoantibodies, applied in the field of biomedicine, can solve the problems of low sensitivity and specificity of the CBA method, difficult to clearly interpret the detection results, and complicated operation process, so as to increase the detection sensitivity and specificity, and interpret the detection results. Easy to identify, reduce the effect of non-specific signal
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[0031] The present invention will be further described in detail below in conjunction with specific embodiments.
[0032] The preparation method of the human anti-AQP4 autoantibody detection material provided by the present invention specifically comprises the following steps:
[0033] 1. Artificially synthesized or obtained AQP4 full-length target gene by PCR method, construct HEK293 / pCI-neo-M1-AQP4, HEK293 / pCI-neo-M23-AQP4 cells stably or transiently expressing M1 and M23 subtypes, and empty them HEK293 / pCI-neo cells were used as the control group. The specific steps are as follows:
[0034] 1. Plasmid construction
[0035] 1.1 Obtaining the target gene: obtain the full-length target gene of AQP4 of M1 and M23 subtypes by artificial synthesis or PCR method, and add NotI / NheI enzyme cutting sites at both ends.
[0036] 1.2 Insert the obtained two target genes into the vector pCI-neo plasmid respectively, the insertion site is NotI / NheI, name the obtained two plasmids respe...
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