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Preparation method of colostrum peptide of proline-rich polypeptides

A proline and colostrum technology, which is applied in the field of biological polypeptide extraction, can solve the problems that the preparation method is not suitable for industrial production, the preparation process is complicated, and the cost is high, achieving the effects of high yield, simple operation steps, and reduced processing costs

Active Publication Date: 2017-08-08
江苏天美健大自然生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The BPLP is also genetically encoded and is not a natural source of bovine colostrum; Chinese patent CN 201310726948.0 discloses a wheat proline-rich protein Tazmh122 and its encoding gene and application. The wheat proline-rich protein is obtained through genetic encoding. Not obtained from natural sources of bovine colostrum; and commercial products: Colostrinin, MemoryAid, CogniSure, Cognate, Cognase, Dyna, it can be seen that although the content of proline-rich polypeptides prepared by chemical synthesis and genetic engineering is high, the activity Good, but its cost is high, the preparation process is complicated, and the cycle is long
According to existing reports, a proline-rich polypeptide is prepared from bovine colostrum by a two-step method (ethanol extraction method, ammonium sulfate precipitation method). Industrial production

Method used

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  • Preparation method of colostrum peptide of proline-rich polypeptides
  • Preparation method of colostrum peptide of proline-rich polypeptides
  • Preparation method of colostrum peptide of proline-rich polypeptides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Prepare bovine colostrum: collect the milk produced by healthy cows within 72 hours after giving birth to obtain bovine colostrum;

[0027] Skimming: Take 100kg of the bovine colostrum prepared above and place it in a continuous disc centrifuge for separation at a temperature of 40°C and a rotating speed of 5500rpm. After centrifugation, 80kg of skimmed milk is obtained;

[0028] Separation of casein: adjust the pH of the skim milk obtained after skimming to 4.5 with 10% acetic acid, keep it warm at 40°C for 30 minutes, and place it in a continuous disc centrifuge for separation, current 30A, speed: 2500r / min, pressure: 0.4 Mpa, to get clarified whey, to get about 8kg of casein;

[0029] Sterilization: The above-mentioned clarified whey is selected from the No. 1 ceramic membrane filtration system produced by the French TAMI company. The ceramic membrane pore size is 0.45 μm, and the microfiltration is sterilized. The pressure is 0.25Mpa, the temperature is 35°C, and th...

Embodiment 2

[0036]Prepare bovine colostrum: collect the milk produced by healthy cows 68 hours after giving birth to obtain bovine colostrum;

[0037] Skimming: Take 100kg of the bovine colostrum prepared above and place it in a continuous disc centrifuge for separation at a temperature of 45°C and a rotating speed of 6000rpm. After centrifugation, 81kg of skimmed milk is obtained;

[0038] Separation of casein: adjust the pH of the skim milk obtained after skimming to 4.2 with 10% acetic acid, keep it warm at 38°C for 25 minutes, place it in a continuous disc centrifuge for separation, current 30A, speed: 2500r / min, pressure: 0.4 Mpa, to get clarified whey, to get about 8.2kg of casein;

[0039] Sterilization: The above-mentioned clarified whey is selected from the No. 1 ceramic membrane filtration system produced by the French TAMI company. The ceramic membrane pore size is 0.45 μm, and the microfiltration is sterilized. The pressure is 0.25Mpa, the temperature is 38°C, and the flow rat...

Embodiment 3

[0046] Prepare bovine colostrum: collect the milk produced by healthy cows 24 hours after giving birth to obtain bovine colostrum;

[0047] Skimming: Take 100kg of the bovine colostrum prepared above and place it in a continuous disc centrifuge for separation at a temperature of 42°C and a rotating speed of 5800rpm. After centrifugation, 85kg of skimmed milk is obtained;

[0048] Separation of casein: adjust the pH of the skim milk obtained after skimming to 4.5 with 10% acetic acid, keep it warm at 38°C for 25 minutes, place it in a continuous disc centrifuge for separation, current 30A, speed: 2500r / min, pressure: 0.4 Mpa, to get clarified whey, to get about 8.5kg of casein;

[0049] Sterilization: The above-mentioned clarified whey is selected from the No. 1 ceramic membrane filtration system produced by the French TAMI company. The ceramic membrane pore size is 0.45 μm, and the microfiltration is sterilized. The pressure is 0.25Mpa, the temperature is 35°C, and the flow ra...

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Abstract

The invention discloses a preparation method of a colostrum peptide of proline-rich polypeptides, which comprises the following steps: collecting colostrum of healthy cows within 72 hours of delivery to obtain bovine coloctrum; performing centrifugal degreasing to obtain skim milk; performing microfiltration sterilization after separating casein from the skim milk, or directly performing microfiltration sterilization on the skim milk; adding protease, and adjusting the pH for enzymolysis at 2 to 5 DEG C; adding an ammonium salt for precipitation at 2 to 5 DEG C, and applying pressure for coagulation to obtain a coagulation product; adding water to the coagulation product to dissolve; performing ultrafiltration concentration; performing freeze drying to obtain the colostrum peptide. The preparation method achieves colostrum peptide industrial production of separating and purifying proline-rich polypeptides from bovine colostrum, improves the economic benefit of a bovine colostrum manufacturer and reduces the waste disposal cost of the manufacturer. The invention also discloses the colostrum peptide of proline-rich polypeptide, the purity is high, proline accounts for the main part, and the content of the proline is about 27%, and the yield is higher.

Description

technical field [0001] The invention belongs to the technical field of biological polypeptide extraction, and in particular relates to a colostrum peptide rich in proline polypeptide and a preparation method thereof. Background technique [0002] Proline-rich-proteins (PRPs) represent a class of structural proteins rich in proline and hydroxyproline, which widely exist in dicotyledonous and monocotyledonous plants. There are many kinds of proline-rich proteins, and the diversity of structures determines the diversity of functions, but there are few domestic studies on them. [0003] In 1974, when Polish scientist Janusz et al. purified goat colostrum IgG, they discovered a substance proline polypeptide (PRP) that could not be precipitated by anti-immunoglobulin antiserum at the same time. Proline-rich polypeptide (PRP) is An immunoactive substance, a polypeptide containing a large amount of proline and hydrophobic amino acids, exists in the blood of animals and colostrum of...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14C07K1/30C07K1/34
CPCC07K1/145C07K1/30C07K1/34C12P21/06
Inventor 王荣昌饶安平郁桦李玲葛文金甘露汪普林
Owner 江苏天美健大自然生物工程有限公司