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Device and method for extracting in situ root exudates of arbuscular mycorrhizal plants

A technology of root exudates and plant roots, which is applied in the field of devices for in-situ extraction of arbuscular mycorrhizal plant root exudates, can solve problems such as differences and difficulty in ensuring integrity, and achieve convenience in use, integrity protection, and simplified operation The effect of steps

Active Publication Date: 2017-08-11
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the integrity of the root system in the process of extracting arbuscular mycorrhizal plant root exudates is difficult to ensure due to the difference between the natural growth conditions of the plant root system and the test conditions, so that the test results are quite different from the normal secretion results of the plant roots. to provide a device and method for in situ extraction of arbuscular mycorrhizal plant root exudates

Method used

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  • Device and method for extracting in situ root exudates of arbuscular mycorrhizal plants
  • Device and method for extracting in situ root exudates of arbuscular mycorrhizal plants
  • Device and method for extracting in situ root exudates of arbuscular mycorrhizal plants

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specific Embodiment approach 1

[0025] Specific implementation mode one: combine figure 1 and figure 2 Describe this embodiment, present embodiment comprises container 1 for substrate and external culture container 2, the top of external culture container 2 is an open end, the inside of external culture container 2 is provided with container 1 for substrate, external culture container 2 and external culture container 2 are used for substrate. A ring cavity 5 is formed between the containers 1, and a nutrient solution is arranged in the ring cavity 5. The substrate container 1 is a container made of a flexible membrane, and the external culture container 2 is a container made of a hard transparent material. The top of the substrate container 1 is processed with a seed placement port 3, and the substrate container 1 is provided with a substrate and a bacterial solution.

[0026] In this embodiment, saline-alkali stress, metal stress, organic pollutant stress or other stress factors are added to the ring cham...

specific Embodiment approach 2

[0029] Specific implementation mode two: combination figure 1 and figure 2 This embodiment will be described. In this embodiment, the bottom of the substrate container 1 is provided with a root penetration mesh 4 . The root system provides an effective positioning effect for the plant root system through the mesh 4 . Other unmentioned content is the same as the first embodiment.

specific Embodiment approach 3

[0030] Specific implementation mode three: combination figure 1 and figure 2 This embodiment will be described. In this embodiment, the root penetration mesh 4 is a nylon mesh. The setting of the nylon mesh is more convenient for plant roots to penetrate. The nylon mesh can also cover the bottom of the substrate container 1 to a certain height. The range of the height is 1 / 4 to 1 / 3 of the height of the substrate container 1 . Other unmentioned content is the same as the specific embodiment 1 or 2.

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Abstract

The invention relates to a device and method for extracting exudates, in particular to a device and method for extracting in situ root exudates of arbuscular mycorrhizal plants. Due to the differences between natural growth conditions and test conditions of plant roots, it is difficult to ensure the integrity of the roots during the extraction of root exudates of arbuscular mycorrhizal plants, and great differences occur between the test results and the results of normal exudates of plant roots. A container for media is arranged in an external culture container in the device, an annular cavity is formed between the external culture container and the container for media, a nutrient solution is arranged in the annular cavity, the top end of the container for media is provided with a seed inlet, and a medium and a bacterial liquid are arranged in the container for media; the method comprises the steps of I, adding seeds; II, inoculating an arbuscular mycorrhiza fungal agent to the container for media; III, performing mycorrhization; IV, extracting root exudates in the annular cavity. The device and method are applicable to the test process of extracting root exudates of arbuscular mycorrhizal plants.

Description

technical field [0001] The invention specifically relates to a device and method for in-situ extraction of arbuscular mycorrhized plant root exudates. Background technique [0002] In 1989, Harley divided mycorrhizae into seven types according to the fungi and plant species participating in the symbiosis and the characteristics of their symbiotic system, namely, arbuscular mycorrhizae, ectomycorrhizae, ectomycorrhizae, berry-like mycorrhizae, Crystal mycorrhizae, heather mycorrhizae and Orchidaceae mycorrhizae. As early as 1900, it was known that endomycorrhizal fungi are the most widely distributed and most closely related to agricultural production. They can produce two typical structures of "vesicle" and "arbuscule" in plant root cells, named For vesicles - arbuscular mycorrhiza. Because some fungi do not produce vesicles in the root, but all form arbuscular branches, they are called arbuscular mycorrhiza for short. [0003] Mycorrhizae are the result of the co-evoluti...

Claims

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Application Information

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IPC IPC(8): G01N1/40A01G9/02
CPCA01G9/02G01N1/4005G01N1/4022G01N2001/4027Y02P60/21
Inventor 范晓旭于佳宋福强
Owner HEILONGJIANG UNIV
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