Tripterygium wilfordii pyrophosphate synthase twcps4 and its application in the preparation of abietane-type diterpenoids

A technology of pyrophosphate synthase and compound, applied in application, enzyme, isomerase and other directions, can solve the problem of unsuccessful preparation of triptolide
CN107058274BActive Publication Date: 2018-02-23CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
Publication Date
2018-02-23

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Abstract

The invention discloses novel common threewingnut root copalyl pyrophosphate synthase TwCPS4 and a coding gene thereof. TwCPS4 plays an importance role in inducing the initial synthesis of a common threewingnut root diterpene compound. Catalysis experiments show that by combining TwCPS4 with radix salviae miltiorrhizae SmkSL1 for use, an abietane diterpene precursor can be synthesized with GGPP as a substrate, and a secondary synthesis route of common threewingnut root diterpene with TwCPS4 involved is verified. The efficiency of the combination of TwCPS4 and radix salviae miltiorrhizae SmkSL1 is higher than that of the combination of SmCPS1 and SmkSL1 in abietane diterpene precursor synthesis.
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Description

technical field

[0001] The invention belongs to the field of biopharmaceuticals, and specifically relates to a key enzyme for diterpene compound synthesis obtained through a genetic engineering method, in particular to tripterygium wilfordii diterpene synthase and its use in the preparation of abietane-type diterpene compounds. Background technique

[0002] The medicinal plant Tripterygium wilfordii.Hook.f. is a Chinese herbal medicine,

[0003] It is widely used in the treatment of rheumatoid arthritis and inflammation (Raphaela G M, Mildred W, Roy F, et al. Comparison of Tripterygium wilfordii Hook F Versus Sulfasalazine in the Treatment of Rheumatoid Arthritis: A Randomized Trial[J]. Annals of Internal Medicine, 2009, 151(4): 229-240. Tao X L, Lipsky P E. The Chinese anti-inflammatory and immunosuppressive herbal remedy Tripterygium wilfordii Hook F. [J]. Rheumatic Disease Clinics of North America, 2000, 26(1): 29 -50.). Terpenes are the main active ingredients of Tript...

Claims

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