Microbial agent for oil-sludge separation and oil-sludge separation method
A microbial agent and oil sludge separation technology, applied in the field of oil sludge separation, can solve the problems of large consumption of chemical reagents, waste of petroleum resources, pollution transfer, etc., and achieve the effects of reducing oil sludge pollution, strong market competitiveness, and stable performance
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Embodiment 1
[0029] The sludge separation method is as follows:
[0030] (1) The microbial agents used in this biotechnology are: a: Bacillus thermodenitrophilus NG80-2; b: Geobacter thermoglucoside w-2.
[0031] (2) The weight percentage of the microbial agent involved in this biotechnology and the oil sludge it acts on is:
[0032] Microbial agent 50% sludge 50%.
[0033] (3) The treatment process and reaction conditions of the biotechnology are:
[0034] Prepare culture solution A and B according to the above ratio, and after autoclaving, culture microorganism a in culture solution A and culture solution B in culture solution B, and seal it; 45°C, 160rpm vortex stirring, culture for 16h; then microorganism a and b are mixed according to 1:1 to make microbial agent; add oil sludge (the oil content in the oil sludge is 51%) and microbial agent in a ratio of 1:1, and seal it. 45°C, vortex stirring at 160 rpm, and react for 20 minutes. After the reaction, let it stand still, and after t...
Embodiment 2
[0038] The sludge separation method is as follows:
[0039] (1) The microbial agents used in this biotechnology are: a: Bacillus thermodenitrophilus NG80-2; b: Geobacter thermoglucoside w-2.
[0040] (2) The weight percentage of the microbial agent involved in this biotechnology and the oil sludge it acts on is:
[0041] Microbial agent 50% sludge 50%.
[0042](3) The treatment process and reaction conditions of the biotechnology are:
[0043] Prepare culture solution A and C according to the above ratio, and after autoclaving, culture microorganism a in culture solution A and culture solution b in culture solution C, and seal it; 70°C, 130rpm vortex stirring, culture for 16h; then microorganism a and b are mixed according to 1:1 to make microbial agent; add oil sludge (the oil content in the oil sludge is 51%) and microbial agent in a ratio of 1:1, and seal it. 70°C, vortex stirring at 130rpm, and react for 1h. After the reaction, let it stand still, and after the oil and...
Embodiment 3
[0047] The sludge separation method is as follows:
[0048] (1) The microbial agents used in this biotechnology are: a: Bacillus thermodenitrophilus NG80-2; b: Geobacter thermoglucoside w-2.
[0049] (2) The weight percentage of the microbial agent involved in this biotechnology and the oil sludge it acts on is:
[0050] Microbial agent 50% sludge 50%.
[0051] (3) The treatment process and reaction conditions of the biotechnology are:
[0052] Prepare culture solution B and C according to the above ratio, and after autoclaving, culture microorganism a in culture solution B and culture solution B in culture solution C, and seal it; 50°C, 160rpm vortex stirring, culture for 16h; then microorganism a and b are mixed according to 1:1 to make microbial agent; add oil sludge (the oil content in the oil sludge is 51%) and microbial agent in a ratio of 1:1, and seal it. 50°C, vortex stirring at 160rpm, and react for 2h. After the reaction, let it stand still, and after the oil an...
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