Unlock instant, AI-driven research and patent intelligence for your innovation.

Microbial agent for oil-sludge separation and oil-sludge separation method

A microbial agent and oil sludge separation technology, applied in the field of oil sludge separation, can solve the problems of large consumption of chemical reagents, waste of petroleum resources, pollution transfer, etc., and achieve the effects of reducing oil sludge pollution, strong market competitiveness, and stable performance

Active Publication Date: 2017-08-29
NANKAI UNIV
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these existing processing methods have some obvious disadvantages
Such as the disadvantages of the chemical separation method: the separation efficiency is not high, the consumption of chemical reagents is large, the treatment is not thorough, and there is still a lot of pollution in the separated water and mud sand, which still needs to be repeatedly treated to achieve the target; Occupy a large amount of valuable fields and cause pollution transfer; the reinjection method is limited by the site and cannot completely seal the leakage of petroleum-based harmful substances and derivative chlorides in the oily sludge; the decomposition method is too expensive to operate and difficult to operate. The chemical decomposition agent will also cause secondary pollution; the incineration law wastes a lot of precious oil resources, does not conform to the principle of energy conservation and easily pollutes the air
Therefore, the above-mentioned several traditional methods cannot well solve the problem of sludge waste disposal.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial agent for oil-sludge separation and oil-sludge separation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The sludge separation method is as follows:

[0030] (1) The microbial agents used in this biotechnology are: a: Bacillus thermodenitrophilus NG80-2; b: Geobacter thermoglucoside w-2.

[0031] (2) The weight percentage of the microbial agent involved in this biotechnology and the oil sludge it acts on is:

[0032] Microbial agent 50% sludge 50%.

[0033] (3) The treatment process and reaction conditions of the biotechnology are:

[0034] Prepare culture solution A and B according to the above ratio, and after autoclaving, culture microorganism a in culture solution A and culture solution B in culture solution B, and seal it; 45°C, 160rpm vortex stirring, culture for 16h; then microorganism a and b are mixed according to 1:1 to make microbial agent; add oil sludge (the oil content in the oil sludge is 51%) and microbial agent in a ratio of 1:1, and seal it. 45°C, vortex stirring at 160 rpm, and react for 20 minutes. After the reaction, let it stand still, and after t...

Embodiment 2

[0038] The sludge separation method is as follows:

[0039] (1) The microbial agents used in this biotechnology are: a: Bacillus thermodenitrophilus NG80-2; b: Geobacter thermoglucoside w-2.

[0040] (2) The weight percentage of the microbial agent involved in this biotechnology and the oil sludge it acts on is:

[0041] Microbial agent 50% sludge 50%.

[0042](3) The treatment process and reaction conditions of the biotechnology are:

[0043] Prepare culture solution A and C according to the above ratio, and after autoclaving, culture microorganism a in culture solution A and culture solution b in culture solution C, and seal it; 70°C, 130rpm vortex stirring, culture for 16h; then microorganism a and b are mixed according to 1:1 to make microbial agent; add oil sludge (the oil content in the oil sludge is 51%) and microbial agent in a ratio of 1:1, and seal it. 70°C, vortex stirring at 130rpm, and react for 1h. After the reaction, let it stand still, and after the oil and...

Embodiment 3

[0047] The sludge separation method is as follows:

[0048] (1) The microbial agents used in this biotechnology are: a: Bacillus thermodenitrophilus NG80-2; b: Geobacter thermoglucoside w-2.

[0049] (2) The weight percentage of the microbial agent involved in this biotechnology and the oil sludge it acts on is:

[0050] Microbial agent 50% sludge 50%.

[0051] (3) The treatment process and reaction conditions of the biotechnology are:

[0052] Prepare culture solution B and C according to the above ratio, and after autoclaving, culture microorganism a in culture solution B and culture solution B in culture solution C, and seal it; 50°C, 160rpm vortex stirring, culture for 16h; then microorganism a and b are mixed according to 1:1 to make microbial agent; add oil sludge (the oil content in the oil sludge is 51%) and microbial agent in a ratio of 1:1, and seal it. 50°C, vortex stirring at 160rpm, and react for 2h. After the reaction, let it stand still, and after the oil an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a microbial agent for oil-sludge separation and an oil-sludge separation method. The oil-sludge separation method includes: preparing a culture solution A-C, sterilizing at high pressure, respectively adding microorganisms a and b solely, and sealing; performing vortex stirring at 40-70 DEG C and 100-200rpm, and culturing for 16h; mixing the microorganisms a and b according to a ratio of 1:1 to obtain the microbial agent; mixing the microbial agent with oil-sludge according to a ratio of 1:1 and 2:1, performing vortex stirring at 40-70 DEG C and 100-200rpm, and allowing reaction for 20min-2h; standing after reaction is finished to obtain oil-sludge separated by the microbial agent after oil and soil are layered, wherein the microorganism a is bacillus denitrification thermophilus, and the microorganism b is thermal glucoside soil bacillus w-2. The oil-sludge separation method is simple in process and needed equipment, low in cost, environment-friendly and capable of treating series oil-sludge different in oil content (20-60%) according to needs, sandy soil after separation is low in oil content and clean, and the oil-sludge separation method is novel biotechnology for oil-sludge separation and has good application prospect.

Description

technical field [0001] The invention belongs to the technical field of oil sludge separation, and relates to a biotechnology and application of oil sludge separation, more specifically, a method and application of oil sludge separation using microbial bacterial agents. Background technique [0002] In the process of oil exploitation, storage and transportation, and refining and processing, some sludge with high oil content is often produced. If these sludge is piled up randomly, it will become an important source of pollution in the oil field and the surrounding environment. After oil enters the soil, it is difficult to remove, which not only destroys the soil structure, but also causes harm to the soil's own microorganisms and soil plant ecosystem. [0003] At present, the treatment methods for sludge waste generally include: chemical separation, decomposition, reinjection, landfill or incineration. However, these existing processing methods all have some obvious disadvant...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/20
Inventor 王威时威威郭姝怡
Owner NANKAI UNIV