Anthurium andraeanum body cell cross breeding method based on protoplast asymmetric fusion technology

Abstract

The invention belongs to the technical field of plant cross breeding, and discloses an anthurium andraeanum body cell cross breeding method based on a protoplast asymmetric fusion technology. The method comprises the following steps: inoculating a disinfected tender leaf or root tip of an anthurium andraeanum tender during the growth onto a culture medium, so as to culture and form callus tissue, and then transferring the disinfected tender leaf or root tip into a liquid induction medium to perform shaking suspension culture, so as to obtain a suspension cell line; inoculating the suspension cell line into a liquid medium containing enzymatic hydrolysate, so as to perform enzymolysis treatment and obtain protoplast, treating donor protoplast through colchicines, and then performing induction through a PEG high ca<2+>-high pH value method, so as to perform protoplast fusion; and transferring a fusion product into a protoplast liquid medium, and transferring an obtained cell cluster onto an induction medium to culture the cell cluster, so as to obtain a complete body cell hybrid plant. The method is simple and easy and high in fusion frequency, can greatly broaden the genetic diversity of anthurium andraeanum, and develops a novel approach for the cross breeding, genetic improvement and new germplasm development of anthurium andraeanum.

Description

technical field [0001] The invention belongs to the technical field of plant cross-breeding, and in particular relates to an anthurium somatic cell cross-breeding method based on protoplast asymmetric fusion technology. Background technique [0002] Anthurium andraenum (Anthurium andraenum) is a perennial herbaceous plant of the Araceae family Anthurium. Its origin is Colombia. It is currently the world's best-selling ornamental plant with both cut flowers and foliage potted plants. It has high economic value and broad market development prospects. At present, almost all potted and cut flower varieties in the market are mostly selected from the interspecific crossing of Anthurium Schott or intraspecific hybridization of A.andraeanum Lind, creating rich genetic variation. After decades of cultivation, professional companies such as Anthurium and Ryan in the Netherlands and research institutions such as the University of Hawaii and the University of Florida in the United State...

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Problems solved by technology

[0003] In summary, the existing problems in the prior art are: traditional crossbreeding has a long cycle of anthurium crossbreeding, which usually takes 7-8 years; there are generally reproductive obstacles such as self-breeding or hybridization incompatibility, and decline of inbred offspring ; There are harmfulness and randomness of anthurium species mutation in mutation breeding, and the purpose of radiation breeding is not strong; the ploidy breeding of anthurium is often accompanied by the reduction of anthurium agronomic traits and ornamental traits, to a certain extent Influenced the wide application of polyploid breeding of Anthurium

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