Method for the management of biology in a batch process
A technology of microorganisms and species, applied in the field of management biology, can solve problems such as unclear
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Embodiment 1
[0074] The efficiency of the methanogenic microorganisms used in the method of the present invention is measured by the methane production rate of the methanogenic culture. The applicant expects that the methane production rate is about 0.12 grams of chemical oxygen demand (COD) per gram of volatile solids per day (ie, 0.12 g COD / g VS / d). It is also possible to infer the efficiency of hydrogen-consuming methanogens by maintaining the hydrogen concentration in the biogas below 0.01% (10 ppm) and effectively removing volatile fatty acids, especially acetate and propionate.
[0075] The preferred biological parameters of anaerobic digestion are as follows:
[0076] (i) The pH is maintained between about 6.0 and 8.5, such as between 6.5 and 7.5;
[0077] (ii) The oxidation-reduction potential (ORP) is maintained below about -180mV, such as -280mV;
[0078] (iii) Ammonia (total ammonium nitrogen or "TAN") is maintained below about 3,000 mg / L, such as about 2,000 mg / L;
[0079] (iv) The ele...
Embodiment 2
[0086] Describe the microbial community through terminal restriction fragment length polymorphism (T-RFLP)
[0087] T-RFLP is a molecular method that allows relatively rapid exploration of microbial communities, and the community data profiles can be compared in samples collected at different time points. Extract DNA from the sample, use a fluorescently labeled primer pair to selectively amplify the 16S gene, and use a restriction nucleic acid that is cut with a specific sequence (using 4bp enzyme, because they cut most frequently, every 256bp) Dicer digests the PCR product, and the digested PCR product runs on a capillary sequencer. The capillary sequencer allows the end-labeled fragments to be accurately sized. The resulting data chart can provide information on microbial identification (fragment size) and abundance (peak area).
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