Phenolic amide compound II and extraction method and application thereof
A compound, phenolamide technology, applied in the field of traditional Chinese medicine extraction, can solve the problems of extracting phenolamide compounds, the extraction method and use have not yet been reported, etc.
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Embodiment 1
[0052] The extraction method of phenolic amide compound II is characterized in that comprising the steps:
[0053] (1) With the dry persistent calyx and fruit (4.5kg) of Physalis physalis as raw material, add the volume fraction of 12 mass times of raw material and be 70% ethanol aqueous solution, reflux extraction 2 times, extract 2 hours each time, merge and obtain extract, reduce Pressure reclaims solvent, obtains total extract (550g) after concentrating;
[0054] (2) Disperse the total extract into 5 times by mass of water, extract with sherwood oil and ethyl acetate successively, and recover the solvent under reduced pressure from the ethyl acetate extract to obtain the ethyl acetate layer extract (102g);
[0055] (3) The extract from the ethyl acetate layer was separated by silica gel column chromatography, and the dichloride dichlorides with volume ratios of 100:1, 100:2, 100:5, 100:10, 5:1, 2:1 and 1:1 were respectively Gradient elution with methane-methanol as the el...
Embodiment 2
[0073] The extraction method of phenolic amide compound II comprises the steps:
[0074] (1) Using the dried persistent calyx and fruit of Physalis pseudophysalis as raw material, add 80% ethanol aqueous solution with a volume fraction of 8 mass times of the raw material, reflux extraction 3 times, extract for 2 hours each time, combine to obtain the extract, and recover the solvent under reduced pressure, Obtain the total extract after concentration;
[0075] (2) Disperse the total extract into water of 5 times by mass, extract with equal volumes of petroleum ether and ethyl acetate successively, and recover the solvent under reduced pressure from the ethyl acetate extract to obtain the ethyl acetate layer extract;
[0076] (3)-(9) are the same as embodiment 1(3)-(9).
Embodiment 3
[0078] The extraction method of phenolic amide compound II comprises the steps:
[0079] (1) Use dried calyx and fruit of pseudophysalis as raw material, add 60% ethanol aqueous solution with a volume fraction of 10 times the mass of the raw material, reflux extraction 3 times, extract 2 hours each time, combine to obtain the extract, recover the solvent under reduced pressure, and concentrate After obtaining the total extract;
[0080] (2) Disperse the total extract into 10 mass times of water, extract with equal volumes of petroleum ether and ethyl acetate successively, and recover the solvent under reduced pressure from the ethyl acetate extract to obtain the ethyl acetate layer extract;
[0081] (3)-(9) are the same as embodiment 1(3)-(9).
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