Preparation method of anthocyanin standard

A standard product, anthocyanin technology, applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., to achieve the effects of strong growth and reproduction, improved separation and purification efficiency, and easy access

Active Publication Date: 2017-09-01
丁新华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Currently, there is no preparation of Petunidin-3-(trans-p-coumaroyl)-rutinoside-5-glucoside (Petunidin-3-(trans-p-coumaroyl)-rutinoside-5-glucoside) standard information, while petunidin-3-trans-p-coumaroylrutinoside-5-glucoside (Petunidin-3-( trans - p -coumaroyl) -rutinoside-5-glucoside) standard products have not been reported

Method used

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  • Preparation method of anthocyanin standard
  • Preparation method of anthocyanin standard
  • Preparation method of anthocyanin standard

Examples

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Embodiment

[0035] The preparation method of a kind of anthocyanin standard product petunia-3-trans-p-coumaroylrutinoside-5-glucoside of the present embodiment comprises the following steps:

[0036] (1) Refer to the invention patent application (Application No. 201510948177.9) titled "A Method for Extracting High-Purity Anthocyanins" to obtain Solanum Solanum Anthocyanin powder with a purity higher than 50%. Take 500mg of Anthocyanin powder through After 10ml of 3% acetic acid is fully dissolved, filter through filter paper to remove insoluble solid impurities.

[0037] (2) Shake and mix the filtered anthocyanin solution with an equal volume of ethyl acetate, shake once every 2 hours, and extract in the dark for 12 hours, discard the upper ethyl acetate layer after each extraction, and repeat 4 times , fully extract the flavonoid impurities. Such as figure 1 As shown, the upper layer is dark yellow in color of flavonoid impurities extracted by ethyl acetate, and the lower layer is dar...

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Abstract

The invention discloses a preparation method of an anthocyanin standard, namely petunidin-3-(trans-p-coumaroyl)-rutinoside-5-glucoside. The preparation method comprises the steps of extraction and purification of polydextran gel resin and a preparation liquid phase. According to the method, an extraction source of anthocyanin is a nightshade fruit; the nightshade fruit is easy to obtain; a proportion of the anthocyanin standard in the anthocyanin of the nightshade fruit is maximum; and the extraction cost of the anthocyanin standard is greatly lowered. At the same time, the polydextran gel resin and the preparation liquid phase are separated, purified and collected in an extraction process; the separation and purification efficiency can be significantly improved; and at last, a petunidin-3-(trans-p-coumaroyl)-rutinoside-5-glucoside standard with purity of greater than 98% can be obtained.

Description

technical field [0001] The invention relates to the technical field of extraction of natural active substances, in particular to a method for preparing anthocyanin standard products. Background technique [0002] As a natural pigment, anthocyanins are water-soluble and give many flowers and fruits their purple, blue and red colors, while promoting pollination and seed distribution. Many investigations have confirmed that natural anthocyanins can protect plants from biotic and abiotic stresses. For example, anthocyanins can provide plant resistance to some fungal diseases and insect pests. In addition, anthocyanins are able to protect plants from cold damage and UV rays. In addition to the physiological effects on plants, a healthy diet rich in anthocyanins also has several significant health-promoting effects. Anthocyanins can prevent certain chronic diseases including high blood sugar and inhibit the growth of tumor cells, and can also improve vision. Due to these benef...

Claims

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Application Information

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IPC IPC(8): C07H17/065C07H1/08
CPCC07H1/08C07H17/065
Inventor 丁新华储昭辉汪少丽赵长宝费丹
Owner 丁新华
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