Novel method for preparing nano roughened PDMS (Polydimethylsiloxane) substrate
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A new method, nanotechnology, applied in biochemical equipment and methods, bone/connective tissue cells, coatings, etc., can solve problems such as roughness effects
Active Publication Date: 2017-09-01
SOUTHWEST UNIV
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At present, many scholars have studied the method of changing its hydrophobicity, and there are many examples with obvious effects, but the influence of the interaction of these methods on its roughness remains to be studied
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Embodiment 1
[0022] Example 1 Preparation of PDMS substrates with different nano-roughness
[0023] (1) Prepare the mold: use ethanol as the solvent, prepare acetone solutions with volume fractions of 25%, 50%, 75%, and 100%, respectively, pour these different concentrations of acetone into 24-well plates, and add 1mL to each well , and the action time was 1 min, 3 min, 6 min, and 10 min, respectively, thus producing 16 culture plate bases with different concentrations and action times.
[0024] (2) Preparation of PDMS with different nano-roughness: Mix the elastic agent and curing agent at a ratio of 10:1, stir evenly, then pour them into 16 different molds, remove air bubbles in vacuum for 1 hour, and then heat at 70 degrees for 2 hours , and then tear off the PDMS from the 24-well plate, and then use the elastic agent and the curing agent to configure the PDMS with a mass ratio of 10:1 to reversely adhere the 16 PDMS with different nanometer roughness to the new 24-well plate ,stand-by...
Embodiment 2
[0026] Example 2 Culture of mesenchymal stem cells on PDMS with different nano-roughness
[0027] (1) The mesenchymal stem cells were inoculated on two groups of PDMS substrates with different nano-roughness, respectively: 50% (1 min, 3 min, 6 min, 10 min) and 3 min (25 min). %, 50%, 75%, 100%) to test the cell adhesion after 4 hours, the results are as follows Figure 4 . The results showed that the greater the roughness, the greater the amount of cell adhesion. In addition, the activity of the cells was tested on the third day, the sixth day and the tenth day, and the results were as follows: Figure 5 , the results showed that with the increase of roughness, the cell growth was promoted gradually.
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Abstract
The invention relates to surface modification of biological materials and more specifically relates to a method for preparing a polystyrene mold with different roughness based on active chemical properties of acetone and rapidly molding a PDMS (Polydimethylsiloxane) substrate with nano roughness by utilizing the mold, wherein the acetone is mainly used as a solvent, is used in industries including explosive, plastic, rubber, fibers, leather making, grease, paint spraying and the like and has an etching effect on a cell culture plate (which is made of a machining material polystyrene). The roughness of the PDMS substrate prepared by the method has a large range and is accurate and controllable. By utilizing a nano roughened PDMS interface, influences, caused by interfaces with the different roughness, on adhesion and proliferation of mesenchymal stem cells are researched; the nano roughened PDMS interface proves that the growth of the mesenchymal stem cells can be effectively promoted by a nano roughened surface.
Description
technical field [0001] The invention relates to the field of biological materials, in particular to exploring the relationship between the nano-roughness and hydrophobicity of PDMS and its influence on the adhesion and proliferation of mesenchymal stem cells. Background technique [0002] Polydimethylsiloxane (Polydimethylsiloxane, PDMS, chemical structure: (C 2 h 6 OSi)n, CAS No.: 9016-00-6) Colorless or light yellow liquid, odorless, high transparency, heat resistance, cold resistance, small viscosity change with temperature, water resistance, small surface tension, thermal conductivity, Good light transmission, excellent physical properties, can be directly used in moisture-proof insulation, damping, shock absorption, defoaming, lubrication, polishing, etc., widely used as insulation lubrication, shockproof, oil dustproof, dielectric and heat carrier . As well as microfluidic channel systems, caulking agents, contact lenses and daily chemical additives. [0003] Becau...
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