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Zinc-enriched yeast product and production method thereof

A technology of zinc-enriched yeast and a production method, which is applied in the field of feed additives, can solve the problems of complex production process, low biological titer of inorganic zinc and high price

Inactive Publication Date: 2017-09-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the domestic use of high-dose zinc oxide, zinc sulfate and other inorganic zinc as zinc nutritional additives, but the biological potency of inorganic zinc is low, the amount of addition is large, and it is easy to cause serious environmental pollution; while the inorganic zinc has good stability and low biological potency. High amino acid complex zinc salts (such as zinc methionine, zinc gluconate, etc.) limit their application due to their complex production process and high price

Method used

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  • Zinc-enriched yeast product and production method thereof
  • Zinc-enriched yeast product and production method thereof
  • Zinc-enriched yeast product and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Yeast strains with different biochemical characteristics are inoculated into media with different zinc concentrations for cultivation, and yeast strains with strong zinc resistance are screened out as mutagenic strains.

[0025] 2) inoculating yeast strains with strong zinc resistance to different doses of nitroso medium for mutagenesis;

[0026] 3) Separating and screening the mutagenized strains, inoculating them into culture media with different zinc concentrations, and screening out strains with higher biomass and strong zinc resistance, which are high-zinc-resistant and zinc-rich yeast strains;

[0027] 4) Screening medium and culture conditions

[0028] Maltose medium, the total sugar content is 5-20%, the zinc (inorganic zinc salt) content is 20-500 μg / ml, the pH value is 4.0-7.0, the culture temperature is 25-36 ° C, and the culture time is 20-45 hours.

Embodiment 2

[0030] Zinc-rich yeast production process: slant culture → primary liquid seed cultivation → secondary liquid seed cultivation → fermenter cultivation → separation and drying → crushing → zinc-enriched yeast products.

[0031] 1) Cultivation of slant strains: Zinc-rich yeast strains are inoculated on wort solid medium with a sugar content of 5-20%, a zinc (inorganic zinc salt) content of 20 μg / ml, and a pH value of 4.0-7.0. Cultivate at 36°C for 20-45 hours.

[0032] 2) First-level liquid seed culture: inoculate slant yeast strains into 50 wort medium containing 250 ml of sugar content of 5-20%, zinc (inorganic zinc salt) 20 μg / ml, and pH value of 4.0-7.0 In the Erlenmeyer flask, the culture temperature is 25-36°C, and the culture is 20-45h.

[0033] 3) Secondary liquid seed culture: Inoculate the primary liquid seed culture solution in 50-100 L of wort with 5-20% sugar content, 20 μg / ml zinc (inorganic zinc salt) and pH 4.0-7.0 In a base fermenter, the culture temperature i...

Embodiment 3

[0038] Zinc-rich yeast production process: slant culture → primary liquid seed cultivation → secondary liquid seed cultivation → fermenter cultivation → separation and drying → crushing → zinc-enriched yeast products.

[0039] 1) Cultivation of slant strains: Zinc-rich yeast strains are inoculated on wort solid medium with a sugar content of 5-20%, a zinc (inorganic zinc salt) content of 500 μg / ml, and a pH value of 4.0-7.0. Cultivate at 36°C for 20-45 hours.

[0040] 2) First-level liquid seed culture: Inoculate slant yeast strains into 50 wort medium containing 250 ml of sugar content of 5-20%, zinc (inorganic zinc salt) 500 μg / ml, and pH value of 4.0-7.0. In the Erlenmeyer flask, the culture temperature is 25-36°C, and the culture is 20-45h.

[0041] 3) Secondary liquid seed culture: Inoculate the primary liquid seed culture solution in 50-100 L of wort with 5-20% sugar content, 200 μg / ml zinc (inorganic zinc salt) and pH value 4.0-7.0 In a base fermenter, the culture tem...

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Abstract

The invention discloses a zinc-enriched yeast product and a production method thereof. The zinc-enriched yeast product has the zinc content of 1000-5000mg in yeast per kg. The production method comprises the following steps: preparing high-zinc-resistant yeast species; sequentially performing slant strain culture, primary liquid seed culture, secondary liquid seed culture, fermentation tank culture, separating, drying, grinding, thereby obtaining the zinc-enriched yeast product. The zinc-enriched yeast product disclosed by the invention does not have any influence on growth performances of growing-finishing pigs and zinc element deposition in tissues, and the zinc excretion in excrements of the growing-finishing pigs can be obviously reduced, so that the zinc-enriched yeast product is proved to have effectiveness on healthy growth of the pigs and environmental protection.

Description

technical field [0001] The invention belongs to the technical field of feed additives, and in particular relates to a zinc-rich yeast product used for animal feed and a production method thereof. Background technique [0002] Zinc is an essential trace element for animal organisms, known as the "element of life". Zinc can promote animal growth and development, affect bone formation, and maintain immune function. The average content in mammals and birds is 30mg / kg. Zinc participates in the regulation of the function, growth and development of almost all immune cells in the body, and is found in more than 200 enzymes required for metabolism in the body. For example, in animal husbandry production, insufficient or poor absorption of zinc in the diet will affect the protein metabolism of the animal and lead to a decrease in animal feed intake, which will cause diarrhea, poor growth, increased feed consumption per unit weight gain, and unsound bone development, etc. A variety o...

Claims

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Application Information

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IPC IPC(8): C12N15/01C12N1/18A23K10/16C12R1/865
CPCC12N1/18C12N15/01
Inventor 冯杰丁成李海云
Owner ZHEJIANG UNIV