A method for rapid determination of wax content in pear fruit epidermis

Abstract

The invention discloses a method for rapidly determining epicuticular wax content of pear fruits. According to the epicuticular wax content determination of the pear fruits in the invention, a 'whole fruit soaking' manner is changed into a 'quantitative peel soaking' manner, the epicuticular wax content is rapidly calculated according to a relationship coefficient between the two, and the superficial area of the pear does not need to be estimated. Liquid transfer does not need to be performed in the operating process, reagents are saved to the greatest degree, time is saved, the operation is simple and convenient, the harm of toxic reagents on experimenters is reduced, and rapid epicuticular wax content determination of the pear fruits can be realized.

Description

technical field [0001] The invention belongs to the technical field of measuring the wax content of plant epidermis, in particular to a method for rapidly measuring the wax content of pear fruit epidermis. Background technique [0002] The waxy layer is a layer of hydrophobic protective substance covering the surface of plants. It acts as the first barrier for plants to contact with the outside world. The morphology of plant leaves, fruit and pollen development also have important influences. In addition, waxes can also be used as important raw materials for paints, lubricants, medicines, cosmetics, and biofuels. Some terpenoids contained in them contain ingredients that are beneficial to human health, and have anti-inflammatory and anti-tumor functions. Therefore, the research on plant wax has potential health benefits and economic value, and wax is becoming one of the important contents of plant research. my country has a long history of pear cultivation and abundant res...

AI Technical Summary

Problems solved by technology

However, the fruit shapes of different varieties of pears are quite different, such as oval, round, gourd, etc., using the same formula to estimate the surface area of ​​all pears, the experimental error is relatively large;

[0005] (2) Too much chloroform is difficult to evaporate, and too little will affect the completeness of wax extraction

Therefore, it takes a lot of organic solvent to measure the wax content of pear fruit skin, and it takes a long time to distill and dry a large amount of organic solvent, and too much solvent needs to be quickly distilled by instruments such as a rotary evaporator, which leads to a large amount of organic solvent. During the extraction process, the waxy extract needs to be transferred multiple times, resulting in waste of medicines, increased time, increased test costs, and increased experimental errors

Trichloromethane extracts plant epidermis wax. Although the effect is good, it is a toxic reagent. Prolonged exposure to trichloromethane during the test will easily cause great harm to the health of the test personnel and the environment.

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