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TLC (Thin layer chromatography) identification method for cacumen biotae

A technology of arborvitae leaves and thin-layer chromatography, which is applied in the field of pharmaceutical analysis, can solve the problems of not being able to meet the needs of quickness and simplicity, the amount of organic reagents used, and the long operation time, so as to reduce discomfort and risk, and reduce identification time The effect of shortening and simple operation

Inactive Publication Date: 2017-09-05
SHANDONG ACAD OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the 2015 edition of the Chinese Pharmacopoeia contains the current legal TLC identification method for Arborvitae leaves, but due to the Soxhlet extraction step used in this method, it takes at least 6 hours, and the heating and reflux step also takes 1 hour, and the operation is cumbersome. , long operation time, high cost due to large amount of organic reagents, etc., cannot meet the fast and simple needs in practical applications

Method used

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  • TLC (Thin layer chromatography) identification method for cacumen biotae
  • TLC (Thin layer chromatography) identification method for cacumen biotae
  • TLC (Thin layer chromatography) identification method for cacumen biotae

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A method for TLC identification of orientalis leaves, the steps comprising:

[0030] (1) Take 1g of the sample powder to be tested, put it in a stoppered Erlenmeyer flask, add 20ml of 70% methanol, ultrasonicate for 30min, filter, evaporate the filtrate to dryness in a water bath, dissolve the residue with 2ml of methanol, and use it as the test solution;

[0031] (2) Take quercitrin standard substance and add ethanol to prepare a 0.1mg / mL solution as the reference solution;

[0032] (3) According to the 2015 edition of the Chinese Pharmacopoeia Part IV 0502 thin-layer chromatography test, draw 3 μL of the test solution and the reference solution, respectively, on the same silica gel G thin-layer plate, and use acetic acid with a volume ratio of 12:1:1 Ethyl ester-formic acid-water was developed as a developer, taken out, dried in the air, sprayed with 1wt% aluminum trichloride-ethanol solution, and inspected under a 365nm ultraviolet lamp.

[0033] In the chromatogram...

Embodiment 2

[0035] A method for TLC identification of orientalis leaves, the steps comprising:

[0036] (1) Take 0.5g of the sample powder to be tested, put it in a stoppered Erlenmeyer flask, add 10ml of 70% methanol, ultrasonicate for 30min, filter, evaporate the filtrate to dryness in a water bath, dissolve the residue with 2ml of methanol, and use it as the test solution;

[0037] (2) Take quercitrin standard substance and add ethanol to prepare a 0.05mg / mL solution as the reference solution;

[0038] (3) According to the 2015 edition of the Chinese Pharmacopoeia Part IV 0502 thin-layer chromatography test, draw 2 μL each of the test solution and the reference solution, and spot them on the same silica gel G thin-layer plate, and use acetic acid with a volume ratio of 10:1:1 Ethyl ester-formic acid-water was developed as a developer, taken out, dried in the air, sprayed with 1 wt% aluminum trichloride-ethanol solution, and inspected under a 365nm ultraviolet lamp.

[0039] In the chr...

Embodiment 3

[0041] A method for TLC identification of orientalis leaves, the steps comprising:

[0042] (1) Take 1.5g of the sample powder to be tested, put it in a stoppered Erlenmeyer flask, add 30ml of 70% methanol, ultrasonicate for 30min, filter, evaporate the filtrate to dryness in a water bath, dissolve the residue with 2ml of methanol, and use it as the test solution;

[0043] (2) Take quercitrin standard substance and add ethanol to prepare a 0.15 mg / mL solution as the reference solution;

[0044] (3) According to the Chinese Pharmacopoeia Part IV 0502 thin-layer chromatography test, draw 4 μL each of the test solution and the reference solution, and spot them on the same silica gel G thin-layer plate, and use ethyl acetate with a volume ratio of 14:1:1 -Formic acid-water is the developing agent, take it out, dry it in the air, spray it with 1wt% aluminum trichloride-ethanol solution, and put it under a 365nm ultraviolet lamp for inspection.

[0045] In the chromatogram of the t...

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Abstract

The invention relates to a TLC identification method of orientalis leaves. The sample powder to be tested is added with methanol, ultrasonicated for 30 minutes, filtered, the filtrate is evaporated to dryness in a water bath, and the residue is dissolved in methanol to be used as a test solution; thin-layer chromatography, ultraviolet light View under lights. It has the advantages of fast, simple, good reproducibility, clear chromatogram, good separation of each spot, and saving medicinal materials.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to a TLC identification method of orientalis leaves. Background technique [0002] Arborvitae leaves, also known as cypress leaves, cluster cypress leaves, cypresses, cloud cypresses, etc., are a good medicine for treating blood that doctors value. Traditional Chinese medicine believes that cypress leaves are bitter in taste, slightly cold in nature, non-toxic, and enter the heart, liver, and large intestine meridians. It has the functions of cooling blood, stopping bleeding, dispelling internal dampness, and dispelling swelling and poison. It can cure hematemesis, epistaxis, hematuria, bloody dysentery, metrorrhagia, rheumatic pain, cough, burns and other diseases. According to scientific research, Arborvitae leaves contain a large amount of volatile oil. The main components in the oil are arborvitene, fennelone, etc., and the leaves also contain various trace a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 石典花张军戴衍朋孙立立
Owner SHANDONG ACAD OF CHINESE MEDICINE