Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Antibacterial peptide as well as preparation method and application thereof

An antimicrobial peptide and hydrophobic chromatography technology, applied in the field of peptides, can solve the problems of easy degradation of expression products, host bacteria toxicity, and low gene expression

Pending Publication Date: 2017-09-08
ZHANGYE OLYMBEL BIO TECH
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the expression of foreign genes, antimicrobial peptides have problems such as low gene expression, easy degradation of expression products, low activity of expression products, and easy toxicity of antimicrobial peptides to host bacteria.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antibacterial peptide as well as preparation method and application thereof
  • Antibacterial peptide as well as preparation method and application thereof
  • Antibacterial peptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Strain modification

[0032] Ultraviolet mutagenesis was carried out to the recombinant Bacillus subtilis strain by conventional methods, and the strain with a larger inhibition zone (hereinafter referred to as the mutant strain) was screened for future use.

[0033] A large number of Salmonella and a small amount of the above-mentioned mutant strains are mixed and cultivated. After the number of viable bacteria of the mutant strains increases and stabilizes, a small amount of bacterial suspension is inoculated into the pure culture of Salmonella, and the mixed culture is repeated. Under selection, a new strain of Bacillus subtilis with stronger antibacterial activity is produced.

[0034] A genetic stability test was carried out on the above-mentioned new strain of Bacillus subtilis to obtain a genetically stable new strain of Bacillus subtilis (hereinafter referred to as a modified strain).

[0035] 2. Fermentation

[0036] The above modified strains were inocula...

Embodiment 2

[0052] In this example, the antimicrobial peptide is synthesized by chemical synthesis, wherein the ABI 433A automatic peptide synthesizer is used for synthesis. The amino acid sequence of the antimicrobial peptide is: Tyr‐Ile‐Pro‐Gln‐Pro‐Arg‐Pro‐Pro‐Pro‐His‐Pro‐Pro Arg-Leu (SEQ ID NO: 1).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides antibacterial peptide as well as a preparation method and an application thereof. The amino acid sequence of the antibacterial peptide is Tyr-Ile-Pro-Gln-Pro-Arg-Pro-Pro-His-Pro-Arg-Leu. The preparation method of the antibacterial peptide comprises the step of carrying out cation exchange chromatography, hydrophobic chromatography and size exclusion chromatography on fermenting liquid containing the antibacterial peptide, so as to obtain the antibacterial peptide. The antibacterial peptide provided by the invention has good stability and high antibacterial activity.

Description

technical field [0001] The invention relates to a polypeptide, in particular to an antibacterial peptide and its preparation method and application. Background technique [0002] Antimicrobial peptides are a class of polypeptide substances with antibacterial activity. At present, a variety of proteins and polypeptides with antibacterial effects have been found in different animal tissues, which are a class of broad-spectrum antibacterial active polypeptides that exist in organisms and are also important components of the innate immune system. These antimicrobial peptides have the characteristics of strong antibacterial ability, wide antibacterial spectrum, many types, wide range of options, and the target strains are not easy to produce resistance mutations. At the same time, in view of the fact that many microorganisms have developed resistance to antibiotics, antimicrobial peptides are expected to become a new generation of antimicrobial peptides. Clinical antibacterial d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C07K1/18C07K1/20C07K1/16A61L2/16A61P31/04A01P1/00A61L101/46
CPCA61L2/16C07K7/08
Inventor 武刚祝发明刘文庆白杰
Owner ZHANGYE OLYMBEL BIO TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com