A kind of rice seed specific promoter posseed and application thereof
A rice seed and promoter technology, applied in the fields of biotechnology and plant genetic engineering, can solve the problems of resource waste, plant death, breaking metabolic balance, etc., and achieve the effect of changing protein and starch content and improving development speed.
Active Publication Date: 2020-08-11
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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Problems solved by technology
At present, constitutive promoters are widely used, but some problems are gradually exposed in the application. For example, it enables the expression of foreign genes in the whole plant, produces a large amount of foreign proteins or metabolites, and breaks the original metabolic balance of plants. Increase the burden on the plants, affect the shape and normal growth and development of the plants, and even lead to the death of the plants, resulting in unnecessary waste of resources
Method used
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Embodiment 2
[0036] In this example, the inventors of the present application discovered and verified another seed promoter, and found that this promoter has a specific expression period.
[0037] The sequence of the promoter is:
[0038]
[0039]
[0040] Referring to the steps (1)-(4) in the above-mentioned Example 1, the inventors of the present application also performed a similar verification on the promoter.
[0041] That is, step 1, wild-type rice Nipponbare genomic DNA extraction;
[0042] Step 2, the acquisition of the promoter and the construction of the plant expression vector;
[0043] Step 3, obtaining positive transgenic plants;
[0044] Step 4, activity identification of the rice seed-specific promoter.
[0045] The results of the Gus gene test in this embodiment are as follows: Figure 4 shown. The experimental method for the verification experiment in this example is exactly the same as that of Example 1 except that the verified promoter sequence is changed.
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The invention provides a rice seed-specific promoter Posseed and application thereof. The rice seed-specific promoter comprises the DNA sequence shown in SEQ ID No: 1 in the sequence listing. The DNA sequence shown in SEQ ID No: 1 in the sequence listing is derived from Nipponbare rice (Oryza sativa L cv. Nipponbare), which is called Posseed or promoter Posseed herein. The invention also provides expression cassettes, plant expression vectors and host bacteria containing the promoter. Specifically, the present invention applies the above-mentioned promoters to plant transgenic engineering. The promoter provided by the present invention can initiate the expression of foreign genes in plants, and is applicable to any plant, especially capable of driving the specific expression of foreign genes in the seeds of rice plants, so it can be used for cultivating ideal rice varieties, or for biological Reactor research.
Description
technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering, in particular to a rice seed-specific expression promoter and its application. Background technique [0002] A promoter is a DNA sequence located in the upstream region of a gene. In genetic engineering, a promoter is an important component. Promoters commonly used in plant genetic engineering can be divided into three categories according to their mode of action and function: constitutive promoters, which drive the continuous and constant expression of exogenous genes in various developmental stages and tissues of plants, regardless of time and space. Tissue-specific promoters, the expression of the genes regulated by them often only occur in certain specific organs or tissue parts, which can make the expression products of the target genes accumulate in a certain space; inducible promoters , generally do not start transcription or the transcription activity...
Claims
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IPC IPC(8): C12N15/113C12N15/84C12N5/10A01H5/10A01H6/46
CPCC12N15/8234C12N15/8245C12N15/8251
Inventor 李浩杨剑波杨亚春秦瑞英李娟李莉许蓉芳
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI