Method for detecting number of effective sperms in porcine seminal plasma

A technology for effective sperm count and detection method, which is applied to measurement devices, preparation and sampling of samples for testing, etc., can solve the problems of decreased semen quality, unsatisfactory quality, prolonged non-productive days of sows, etc., and achieves a simple calculation method. , the effect of simple operation

Inactive Publication Date: 2017-09-08
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the implementation of artificial insemination in boar studs and farms, especially during the transportation of semen, the quality of semen may decline, and even cause the semen to lose its vitality before insemination, and the quality cannot meet the national standard (GB 23238-2009 and GB / T 25172-2010), some expensive testing equipment (sperm automatic analyzer, density meter, etc.) are difficult to be equipped in general pig farms, but room temperature semen still needs to be checked before use, Judging whether it can continue to be used, otherwise it will lead to the failure of mating of sows, prolong the non-production days of sows, and increase the cost of pig farms

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting number of effective sperms in porcine seminal plasma
  • Method for detecting number of effective sperms in porcine seminal plasma
  • Method for detecting number of effective sperms in porcine seminal plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 (fresh pig semen)

[0032] (1) Test preparation

[0033] see figure 1 1mm。 , In the middle of the counting board, there are 100 counting pools of the same size, the counting pools are small squares, the depth of each counting pool is 0.01mm, and the length and width are 0.1mm.

[0034] Place the counting plate on the constant temperature preheating plate on the microscope stage, and adjust the temperature of the constant temperature preheating plate to 37°C; place a cover glass on the top of the counting cell;

[0035] (2) For the freshly collected pig semen, use the semen collection cup to measure the amount of semen collected this time, which is 260ml;

[0036] (3) Take 0.05ml of fresh pig semen and drop it between the cover glass and the counting tank, so that the fresh pig semen will naturally siphon into the counting tank;

[0037] (4) Adjust the magnification of the eyepiece and objective lens of the microscope to 10 times, observe the image of the...

Embodiment 2

[0046] Example 2 (diluted semen):

[0047] (1) Diluted porcine semen or purchased diluted porcine semen is generally produced, stored and transported in accordance with "Normal Temperature Semen of Breeding Pigs" GB 23238-2009 and "Technical Specifications for Production and Storage of Porcine Normal Temperature Semen" GB / T 25172-2010, with a volume of 80ml ;

[0048] (2) The diluted pig semen or the purchased diluted pig semen shall be inspected according to the content of the present invention before use;

[0049] (3) Preparation before inspection

[0050] The structure of the counting plate is the same as in Example 1.

[0051] Place the counting plate on the constant temperature preheating plate on the microscope stage, and adjust the temperature of the constant temperature preheating plate to 37°C; place a cover glass flat on the counting cell;

[0052] (4) Take 0.05ml of diluted porcine semen and drop it between the cover glass and the sperm counting pool, so that the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for detecting the number of effective sperms in porcine seminal plasma. The method comprises the following operation steps: 1, arranging a counting cell with 100 small checks on a counting plate, wherein the depth of the counting cell is 0.01mm, and the length and width of the counting cell are respectively 0.1m; 2, preheating the counting plate to 37 DEG C, and covering a piece of cover glass on the counting cell; 3, dripping the to-be-detected porcine seminal plasma between the cover glass and the counting cell, and naturally sucking the porcine seminal plasma into the counting cell; and 4, performing microscope detection, and performing statistical analysis on sperm density, sperm motility and the number of effective sperms. The method disclosed by the invention is easy to operate and suitable for field detection of seminal plasma before use in a pig farm without an automatic semen analyzer, and only simple instruments and components need to be equipped; the calculation method in the invention is simple, and the number obtained by dividing the amount of sperms in a single small check by 10 refers to the sperm density during seminal plasma detection; and moreover, the sperm motility is obtained by dividing the amount of sperms making a linear motion in a single small counting cell by the total number of sperms in the single small counting cell, and the number of effective sperms is obtained by multiplying the sperm motility by the total amount of sperms in the to-be-detected porcine seminal plasma.

Description

technical field [0001] The invention belongs to the technical field of artificial insemination of pigs, and in particular relates to a detection process of effective sperm count in pig semen. Background technique [0002] Pig artificial insemination technology has been tried in the pig industry since the 1920s. After nearly a century of development, the technology has basically matured and is accepted by most pig farms at home and abroad. my country has released the national standards of "Normal Temperature Semen of Breeding Pigs" (GB / T23238-2009) and "Technical Specifications for Production and Storage of Pig Normal Temperature Semen" (GB / T 25172-2010), which stipulate the requirements for normal temperature semen of breeding boars , Sampling, test methods, judgment rules and signs, packaging, storage and transportation, etc., have played an important role in the promotion and implementation of pig artificial technology. With the implementation of the national fine breed su...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/10G01N1/28
CPCG01N15/10G01N1/2806G01N2015/1006
Inventor 李庆岗吴义景杨家军
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products