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Microfluidic devices with smooth surfaces for the enrichment of rare cells and biomarkers from biological fluids

A microfluidic device and biofluidic technology, applied in animal cells, fluid controllers, laboratory containers, etc., can solve the problems of low flow rate, reduced fluid volume, and insufficient rapid processing of a large amount of blood

Active Publication Date: 2021-04-27
BEIJING NANOPEP BIOTECH CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, low channels result in a reduced volume of fluid flowing through the channels, which in turn results in very low flow rates
Such a device is not fast enough to process large volumes of blood to be practical and useful

Method used

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  • Microfluidic devices with smooth surfaces for the enrichment of rare cells and biomarkers from biological fluids
  • Microfluidic devices with smooth surfaces for the enrichment of rare cells and biomarkers from biological fluids
  • Microfluidic devices with smooth surfaces for the enrichment of rare cells and biomarkers from biological fluids

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Embodiment Construction

[0063] Improved microfluidic devices for handling rare whole cells (eg, circulating tumor cells) are disclosed. Improvements include 1) smooth surface design in the cell capture module; 2) combined cell and biomarker capture mechanism; and, 3) "multiplex" capture of different biomarkers in separate bands in the cell-free biomarker module .

[0064] In summary, the microfluidic device has two integrated capture modules. The first capture module includes a capture zone with an inner surface. The bottom of the inner surface of the first capture module includes a micrometer (μm) to millimeter (mm) level seabed or riverbed simulated smooth topography that facilitates cell-device inner surface interactions. The above-mentioned smooth shape is simulated by using Gaussian or other smooth mathematical functions. Smooth features create a gentle environment to enhance sample mixing, continuous cell-antibody contact, and rare cell capture. The smooth surface design also reduces the sh...

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Abstract

The present invention discloses microfluidic devices and methods for the enrichment and detection of rare cells such as circulating tumor cells (CTCs) and other biomarkers including proteins and DNA / RNA from biological fluid samples. The device improves upon existing microfluidic devices by optimizing the microfluidic design of its cell capture chamber at the micron level to mimic natural smooth surfaces such as those of the ocean floor or river bed. The smooth surface is characterized by creating a gentle environment and enhancing sample mixing to improve sustained cell-antibody contact and capture of rare cells while reducing shear-induced cell damage. The cell capture module is coated with a cell capture ligand. A second optional module binds acellular biomarkers with high sensitivity, enriching acellular biomarkers 100 to 1000-fold while enriching cellular biomarkers.

Description

technical field [0001] The invention relates to the fields of microfluidic devices, cell detection, cell separation and molecular biomarkers. Background technique [0002] Circulating tumor cells (CTCs) originate from primary tumors and possibly from metastatic sites. Cancer cells spread from the primary tumor into the circulatory system as the first step towards metastasis to distant organs. The average tumor releases an estimated millions of cells into the bloodstream each day. Butler et al., "The Physical and functional behavior of capture antibodies absorbed on polystyrene", " Immunol. Meth. , 150:77-90 (1992). Most CTCs do not survive, but those that do pose a risk to the host organism for further metastatic spread of the cancer. Metastasis is the leading cause of death in cancer patients. For solid tumors, the presence of CTCs in Advanced disease is evident, and most evident when sites of metastatic disease have been established. The identification and characterizat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/07B01L3/00
CPCB01L3/502761B01L2200/0668B01L2300/0851B01L2400/086
Inventor 董义蒋春盛
Owner BEIJING NANOPEP BIOTECH CORP LTD
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