A kind of dextran graft modification process of wheat gluten
A wheat gluten protein graft modification technology, applied in the field of protein modification technology, can solve the problems of glucan grafting difficulty and low grafting rate, improve thermal stability and solubility, and promote grafting rate Effect
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Embodiment 1
[0016] A kind of dextran graft modification process of wheat gluten, its preparation steps are as follows:
[0017] Step (1): Weigh a certain amount of wheat gluten and dissolve it in a PBS solution with a pH of 8.0 so that the protein concentration is 12 mg / L;
[0018] Step (2): According to the ratio of wheat gluten / glucan (W / W) 230%, add dextran for mixing, shake until completely dissolved, according to the ratio of wheat gluten / trehalose (W / W) 600% Add trehalose in a proportion of 100%, shake until completely dissolved;
[0019] Step (3): Freeze-dry the mixture, then place the freeze-dried sample in a sealed desiccator with a saturated KBr solution at the bottom, place it in a thermostat at 58°C for 5 minutes, and then transfer it to a thermostat at 50°C The reaction was continued for 90h, and the reaction was terminated.
Embodiment 2
[0021] A kind of dextran graft modification process of wheat gluten, its preparation steps are as follows:
[0022] Step (1): Weigh a certain amount of wheat gluten and dissolve it in a PBS solution with a pH of 8.0 so that the protein concentration is 12 mg / L;
[0023] Step (2): According to the ratio of wheat gluten / glucan (W / W) of 250%, add dextran for mixing, shake until completely dissolved, according to the ratio of wheat gluten / trehalose (W / W) of 500% Add trehalose in a proportion of 100%, shake until completely dissolved;
[0024] Step (3): Freeze-dry the mixture, place the freeze-dried sample in a sealed desiccator with a saturated KBr solution at the bottom, place it in a 58°C thermostat for 10 minutes, and then transfer it to a 52°C thermostat The reaction was continued for 102h, and the reaction was terminated.
Embodiment 3
[0026] A kind of dextran graft modification process of wheat gluten, its preparation steps are as follows:
[0027] Step (1): Weigh a certain amount of wheat gluten and dissolve it in a PBS solution with a pH of 8.0 so that the protein concentration is 12 mg / L;
[0028] Step (2): Add dextran according to the proportion of wheat gluten / glucan (W / W) of 240% for mixing, shake until completely dissolved, according to the proportion of wheat gluten / trehalose (W / W) of 550% Add trehalose in a proportion of 100%, shake until completely dissolved;
[0029] Step (3): Freeze-dry the mixture, place the freeze-dried sample in a sealed desiccator with a saturated KBr solution at the bottom, place it in a thermostat at 58°C for 8 minutes, and then transfer it to a thermostat at 50°C The reaction was continued for 95h, and the reaction was terminated.
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