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Strawberry test-tube plantlet acclimatizing and transplanting medium and application thereof

A technology for transplanting substrates and test-tube seedlings, which is applied in the fields of application, cultivation, and gardening. It can solve the problems of poor transformation mechanism and slow progress in the promotion and application of virus-free seedlings, so as to improve the survival rate of transplanting, improve the life of growers, The effect of strengthening the stress resistance of strawberries

Inactive Publication Date: 2017-09-19
黔东南民族职业技术学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the serious disconnection in the fields of scientific research, production and distribution, and economic costs, the transformation mechanism is not smooth, and the promotion and application of virus-free vaccines is progressing slowly.

Method used

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  • Strawberry test-tube plantlet acclimatizing and transplanting medium and application thereof
  • Strawberry test-tube plantlet acclimatizing and transplanting medium and application thereof

Examples

Experimental program
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preparation example Construction

[0027] Preferably, the preparation method of medicinal dregs comprises:

[0028] S101: Washing, drying and pulverizing each raw material component, then soaking Ligustrum lucidum seeds and Polygonum cephalum in water, adjusting the pH value of the soaked product to 6.5-6.8, adding water to decoct and extract. Wherein, in adding water for decocting and extracting, the mass of water added is 5-10 times of the total mass of Ligustrum lucidum seeds and Polygonum cephalum, and the extraction time is 80-150 min.

[0029] S102: Mix the remaining raw material components uniformly, and extract with ethanol aqueous solution. Wherein, the mass percent concentration of the ethanol aqueous solution is 15%-20%, the extraction temperature is 60°C-70°C, and the extraction time is 60min-200min.

[0030] S103: Mix the decoction-extracted and ethanol-water-extracted products evenly, then filter and collect the filter residue to obtain medicinal residue. Among them, S103 also includes: microwav...

Embodiment 1

[0039] The invention provides a method for rapid propagation of strawberry virus-free tissue culture, comprising the following steps:

[0040] S201: selecting stolons of strawberry plants as explants, and performing heat treatment and disinfection treatment in sequence.

[0041] S202: peeling off shoot tips from the sterilized explants, and then inducing adventitious buds.

[0042] S203: The induced adventitious buds are multi-generationally subcultured to obtain test-tube plantlets.

[0043] S204: Inoculate the ramets of the test-tube plantlets in the root-inducing medium for rooting culture.

[0044] S205: Transplant the test-tube plantlets after rooting culture into the test-tube plantlet acclimatization and transplanting matrix, then manage and cultivate them for 50 days, and after transplanting for 10 days, spray the leaves with nutrient solution twice a week; the whole process of management and cultivation , Spray insect repellant every 15 days, and finally get strawberr...

Embodiment 2

[0047] The invention provides a method for rapid propagation of strawberry virus-free tissue culture, comprising the following steps:

[0048] S201: selecting stolons of strawberry plants as explants, and performing heat treatment and disinfection treatment in sequence.

[0049] S202: peeling off shoot tips from the sterilized explants, and then inducing adventitious buds.

[0050] S203: The induced adventitious buds are multi-generationally subcultured to obtain test-tube plantlets.

[0051] S204: Inoculate the ramets of the test-tube plantlets in the root-inducing medium for rooting culture.

[0052] S205: Transplant the test-tube plantlets after rooting culture into the test-tube plantlet acclimatization and transplanting matrix, then manage and cultivate them for 40 days, and after transplanting for 7 days, spray the leaves with nutrient solution twice a week; the whole process of management and cultivation , Spray insect repellant every 10 days, and finally get strawber...

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Abstract

The invention relates to the technical field of plant culture, in particular to a strawberry test-tube plantlet acclimatizing and transplanting medium and application thereof. The medium comprises a mixture of humus and herb residues, wherein the mass ratio of the humus to the herb residues is 1:1, the herb residues are products obtained by digesting Chinese herbal medicine as raw materials, the Chinese herbal medicine comprises scutellaria baicalensis, folium ginkgo, privet seeds, cordate houttuynia, polygonum capitatum and rhizoma polygonati, and the folium ginkgo is adopted as the main medicine. Strawberry plantlets obtained by the acclimatizing and transplanting medium have multiple leaves, the color of the leaves is green, root systems are enriched, entangled roots are good, the transplanting survival rate of strawberry test-tube plantlets can be remarkably increased, the strawberry fruit yield is remarkably increased, and the number of sicken plantlets is decreased sharply; in addition, broad-leaf humus and herb residues are adopted as raw materials, the operation is easy, the cost is low, the medium is of a great significance in farmer household life improvement, and the strawberry production level is improved to a new level.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a substrate for domestication and transplanting of strawberry test-tube seedlings and an application thereof. Background technique [0002] Strawberry is also called raspberry, ground berry, etc., and belongs to Rosaceae, Fragaria, perennial herb. Strawberry fruit is delicious, red and tender, with juicy pulp, special rich fruit aroma, rich in vitamin C, vitamin A, carotene, etc., and has high nutritional and medicinal value. The latest foreign research points out that strawberry contains a kind of amine substance, which has a certain effect on the treatment of leukemia and aplastic anemia. Therefore, strawberry, as a seasonal fruit suitable for all ages, has a high market price and a large demand. [0003] However, strawberry virus disease, as the main disease affecting strawberry production, generally leads to a 30% to 50% reduction in strawberry yield. At present,...

Claims

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Application Information

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IPC IPC(8): A01G9/10A01G1/00A01H4/00
CPCA01H4/00
Inventor 郑晓峰黄刚何宪江付燕万新屏周黎
Owner 黔东南民族职业技术学院
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