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Tomato stem wilt resistant gene mLCB2b and application thereof

A stem blight and tomato technology, applied in the anti-tomato stem blight gene mLCB2b and its application field, can solve problems that do not meet the production concept

Inactive Publication Date: 2017-09-19
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As for how to improve the resistance of tomato to Alternaria, the chemical regulation methods used in traditional agricultural production are obviously not in line with the current green production concept, and it will be a way to improve the resistance of tomato itself by mining resistance genes. long-term effective method

Method used

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  • Tomato stem wilt resistant gene mLCB2b and application thereof
  • Tomato stem wilt resistant gene mLCB2b and application thereof
  • Tomato stem wilt resistant gene mLCB2b and application thereof

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Embodiment Construction

[0039] The present invention is further described below in conjunction with specific embodiment, but protection scope of the present invention is not limited thereto:

[0040] 1. Cloning and preservation of mLCB2b gene

[0041] The mLCB2b gene is a site-directed mutation of the gene LCB2b encoding the subunit of Serine Palmitoyl Transferase (SPT) in Arabidopsis. The main steps are as follows: the total RNA of Arabidopsis wild-type Col-0 was extracted by Trizol method, and the cDNA of LCB2b was obtained by RT-PCR. In order to make the LCB2b gene insert ATCCT after the start codon 126 bases, the method of segmented amplification is adopted when amplifying the gene: first, the front primer F1 (5 '-ATGATTACGATCCCATACCT-3') (sequence shown in SEQ ID NO: 4) and back primer R1 (5'-AGGATCTGGAGATTGTTGGTGCGCC-3', base inserted in italics) (sequence shown in SEQ ID NO: 5), amplified to obtain 131bp DNA Fragment; then, according to the sequence design of the LCB2b gene ORF 127bp to 280b...

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Abstract

The invention discloses protein mLCB2b capable of improving resistance of tomato to stem wilt caused by Alternaria alternata f.sp.Lycopersici. The invention further discloses a gene mLCB2b for encoding the protein. The gene mLCB2b can be applied to improve resistance of the tomato to the Alternaria alternata f.sp.Lycopersici, namely the gene mLCB2b is used for constructing transgenic tomato to improve resistance of the transgenic tomato to the Alternaria alternata f.sp.Lycopersici.

Description

technical field [0001] The invention belongs to the field of biotechnology. It specifically relates to a tomato stem blight resistance gene mLCB2b and its application. Background technique [0002] In recent years, with the expansion of tomato planting area, the occurrence of tomato stem blight has become more and more serious. It mainly harms the stem and fruit of tomato, and seriously affects the yield and quality of tomato. Alternaria alternataf.sp.Lycopersici (AAL) is the pathogen of tomato stem blight, and it mainly poisons tomato tissue cells by producing pathogenic mycotoxin AAL-toxin. As for how to improve the resistance of tomato to Alternaria, the chemical regulation methods used in traditional agricultural production are obviously not in line with the current green production concept, and it will be a way to improve the resistance of tomato itself by mining resistance genes. long-term effective method. [0003] In the article "Study on the Resistance Mechanism ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/10C12N15/54C12N15/11C12N15/82A01H5/00
CPCC12N9/1029C12N15/8282C12Y203/0105
Inventor 汪俏梅邵志勇赵彦婷王建升胡松申陈珊珊
Owner ZHEJIANG UNIV
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