Multi-stage shaking type bioreactor

Abstract

The invention discloses a multi-stage shaking type bioreactor which comprises a multi-stage shaking type platform, a stepped annular disturber, a driving device and a controller, wherein the multi-stage shaking type platform is used for supporting a disposable biological reaction bag; the stepped annular disturber is used for increasing the turbulence grade of a bottom layer culture liquid in a fixed plate support area; the driving device is used for providing a driving force; the controller is used for controlling the driving device; the multi-stage shaking type platform is fixedly connected with the stepped annular disturber; the driving device is arranged at the multi-stage shaking type platform; the control end of the driving device is electrically connected with a corresponding pin of the controller; the multi-stage shaking type platform comprises a polygon-shaped plate and a shaking plate; the stepped annular disturber comprises a plurality of sleeves and multi-stage telescopic cylinders which are coaxially sleeved; adjacent sleeves are connected in a sliding manner and are distributed in an annular manner; the lengths of the sleeves are gradually reduced from inside to outside; the driving device comprises a driving cylinder, a reverse valve and a middle relay for controlling the reverse valve to work. The multi-stage shaking type bioreactor has the beneficial effects that the culture liquid is high in bottom layer turbulence grade and high in gas mass-transfer efficiency, and the productivity expandability of the platform is improved.

Description

technical field [0001] The invention relates to a multistage shaking bioreactor. Background technique [0002] As an emerging technology, biotechnology has shown enormous economic value and social value, and now it mainly includes genetic engineering technology, fermentation engineering, cell engineering, enzyme engineering and protein engineering. These engineering technologies will involve cell metabolism and cell culture no matter from technical research to final scale production. Among them, the research on bioreactor, a key equipment for cell culture, has become one of the important core technologies in biotechnology. [0003] With the development of bioreactor technology, especially the development of single-use technology, disposable bioreactors have been widely used in seed expansion cultivation, small to pilot-scale mammalian cells, plant cells and insect cells. The production of monoclonal antibodies, vaccines and recombinant proteins has gradually expanded to th...

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the problems that the current rocking platform is difficult to provide higher-quality wave-making ability, the turbulence degree of the bottom layer of the culture solution in the support area of ​​the fixed plate is small, and the gas mass transfer efficiency is not high when the current shaking platform is faced with a larger volume of production and application. A multi-stage rocking bioreactor with high turbulence at the bottom of the culture medium and high gas mass transfer efficiency is proposed

Images