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Application of lactobacillus plantarum YS4 in preparation of food or drugs for preventing ulcerative colitis

A technology for ulcerative colitis and Lactobacillus plantarum, applied in the direction of Lactobacillus, the function of food ingredients, bacteria used in food preparation, etc., can solve the problems of slow progress in treatment, lack of specificity, unclear etiology and pathogenesis , to achieve the effect of expanding the scope of application and improving the application value

Inactive Publication Date: 2017-10-03
CHONGQING UNIV OF EDUCATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Human ulcerative colitis is a type 2 helper T cell (Th2)-mediated inflammatory bowel disease whose etiology and pathogenesis remain unclear, and treatment progress is slow and lacks specificity

Method used

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  • Application of lactobacillus plantarum YS4 in preparation of food or drugs for preventing ulcerative colitis
  • Application of lactobacillus plantarum YS4 in preparation of food or drugs for preventing ulcerative colitis
  • Application of lactobacillus plantarum YS4 in preparation of food or drugs for preventing ulcerative colitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1, isolation and identification of Lactobacillus plantarum YS4

[0018] 1. Isolation and purification of lactic acid bacteria

[0019] Collect 10 samples of naturally fermented yak yoghurt from herdsmen's families in Yushu Tibetan Autonomous Prefecture, Qinghai. After each sample is fully stirred with a sterile glass rod, 50 mL is drawn into a sterile centrifuge tube, placed in a food sampling box and brought back to the laboratory , stored in a 4°C refrigerator.

[0020] Take 1 mL of each yak yoghurt sample, and make a 10-fold gradient dilution with sterile saline to 10 -7 . take 10 -5 、10 -6 、10 -7 Each 1mL dilution of the three dilutions was filled with 15mL MRS medium (containing 5% CaCO 3 ) in a plate, mix well, and place in a 30°C incubator to incubate for 72±3h. Each dilution was performed in triplicate. After the colonies are formed, observe their morphological characteristics, pick different colonies with calcium-dissolving circles and inocul...

Embodiment 2

[0032] Example 2, the preventive effect of Lactobacillus plantarum YS4 on oxazolone-induced colitis in mice

[0033]The main materials, instruments and experimental animals used in this embodiment are as follows: Lactobacillus plantarum YS4 (preservation number: CCTCCNO: M2016750), Lactobacillus bulgaricus (China Industrial Microorganism Culture Collection Management Center); Oxazolone (Sigma Company, USA); MPO, NO, GSH, MDA, SOD assay kit (Nanjing Jiancheng Bioengineering Institute); IL-2, IL-10 serum cytokine assay kit (BioLegend, USA); Trizol reagent, OligodT18, RNase, dNTP, MLV Reverse transcriptase (Invitrogen, USA); ROX reference Dye and SYBR Premix Ex Taq II (TAKARA, Japan); RT-PCR primers (Beijing Tiangen Biochemical Technology Co., Ltd.). Biomate3S UV-visible spectrophotometer, A200PCR instrument, LUX multifunctional microplate reader (Thermo Fisher Scientific, USA); Tancon2500PCR gel imager (Shanghai Tianneng Technology Co., Ltd.); ICEN-24R high-speed refrigerated ce...

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Abstract

The invention discloses application of lactobacillus plantarum YS4, of which the preservation number is CCTCC NO: M2016750, in preparation of food or drugs for preventing ulcerative colitis, so that an application range of the lactobacillus plantarum YS4 is widened, and an application value of the lactobacillus plantarum YS4 is improved; moreover, new hope is brought for treatment or health care on the ulcerative colitis.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and relates to the application of plant lactobacillus in preparing food or medicine. Background technique [0002] Yak yogurt is a naturally fermented dairy product in the Tibetan area of ​​the Qinghai-Tibet Plateau. It is rich in nutrients and has the functions of anti-oxidation, lowering cholesterol and regulating immunity. Due to the special natural fermentation conditions, including fermented raw milk, fermentation temperature, fermentation time, fermentation vessel, especially the composition of special fermentation microorganisms, yak yogurt has a special flavor and quality. [0003] Human ulcerative colitis is an inflammatory bowel disease mediated by type 2 helper T cells (Th2). Its etiology and pathogenesis are still unclear, and the treatment progress is slow and lacks specificity. Oxazolone-induced colitis is an IL-4-mediated Th2-type inflammation, and its histologic...

Claims

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Application Information

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IPC IPC(8): A23L33/135A61K35/747A61P1/04A61P1/00
CPCA23L33/135A61K35/747A23V2002/00A23V2400/169A23V2200/32
Inventor 骞宇赵欣易若琨
Owner CHONGQING UNIV OF EDUCATION
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