Microinjection device used for cell medicine precise injection and operation method of microinjection device

Abstract

The invention belongs to the technical field of cell biology study, and relates to a microinjection device used for cell medicine precise injection and an operation method of the microinjection device. A three-dimensional high-precision left displacement table is fixedly installed on a left lifting table, a three-dimensional high-precision right displacement table is fixedly installed on a right lifting table, a light source transmitting device is fixedly installed at the outer side of the left lifting table, optical tweezers are fixedly installed on the left lifting table, a syringe is arranged at the outer side of the right lifting table, an optical fiber microneedle is fixedly installed on the right lifting table, an objective table is installed between the left lifting table and the right lifting table, a microscope is installed above the objective table, during work, the displacement tables control the optical tweezers and the optical fiber microneedle, and therefore after the optical tweezers capture cells, precise injection of the optical fiber microneedle to the cells is achieved. The cell precise medicine injection device has the function of capturing and controlling the single cell and conducting precise medicine injection on the single cell, is high in precision and adjustability, and has a wide application prospect in the aspects of tumor cell precise medical experimental study and single cell microinjection medicine.

Description

technical field [0001] The invention belongs to the technical field of cell biology research, and relates to a microinjection device for precise injection of cell medicine and an operation method thereof. Background technique [0002] Tumor is one of the most important problems that endanger people's health. At present, 1 / 3 of the people in the world suffer from cancer, but 1 / 4 of them die from cancer. Tumor disease is easy to cause blockage and compression in its diseased area, which is caused by the excessive growth of tumor cells, which is similar whether it is a benign tumor or a malignant tumor. The common ones are blockage of esophagus, blockage of blood vessels and compression of nerves. It will affect the organ or tissue where the tumor disease is located in the adjacent organ, and the structure and function of the tumor cell will be destroyed. For example, liver cancer may experience systemic jaundice due to liver cell destruction and intrahepatic bile duct obstruc...

AI Technical Summary

Problems solved by technology

This cell microinjector uses all mechanical methods to control and inject cells. The existing problems are: the diameter of the micropipe transported by the cell transporter is fixed. For cells of different sizes, it is difficult to ensure that the cells will be arranged in a straight line in the transport pipeline. Front transport; cell capture and clamping adopts mechanical control, which cannot move cells flexibly. The method of mechanical control is very easy to damage cells. Mechanical control has the problem of being unable to stably clamp cells of different diameters. The designed clamping device can only be used for a certain The diameter of the cell clamping effect is the best; the patent does not explain the production method of the injection needle. For the microinjection of single cells, the control and precision of the injection should reach the level of soaring. It is very difficult to make high-precision ultra-fine needle tips; in addition, it is also difficult to pack cells tightly in the cell transporter in this patent

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