Application of bumetanide in promoting neural functional reconstruction in recovery period after ischemic stroke

A technology for ischemic stroke and neurological function, applied in the field of biomedicine, can solve problems such as the unknown

Inactive Publication Date: 2017-11-03
THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there is no relevant report about bumetanide promoting axonal regeneration in the recovery period after ischemic stroke

Method used

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  • Application of bumetanide in promoting neural functional reconstruction in recovery period after ischemic stroke
  • Application of bumetanide in promoting neural functional reconstruction in recovery period after ischemic stroke
  • Application of bumetanide in promoting neural functional reconstruction in recovery period after ischemic stroke

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Experimental program
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Embodiment 1

[0021] Studies have shown that endothelin-1 (ET-1) can be used for the screening of neuroprotective drugs for ischemic stroke. Therefore, the present invention uses an ET-1 injection rat model.

[0022] 1. Establishment of animal model: 44 rats were randomly assigned to ischemia group (ISC group, n=12), sham operation group (SHAM group, n=10), sham operation + bumetanide group (SHAM+ BUM group, n=10), ischemia+bumetanide group (ISC+BUM group, n=12); after inhalation anesthesia, ET-1 (sterile saline diluted to 0.5ug / ul), according to The brain anatomy map of the rat was injected with a microsyringe into three sites in each of the cortex and striatum (①AP +2.3 mm, ML+2.5 mm, DV -2.3mm; ②AP +0.7 mm, ML +2.2 mm, DV -2.0 mm ③AP +0.7 mm, ML +3.8 mm, DV -5.8 mm), each point injects 2μl of medicine, total amount is 6μl; about 2h after injection of ET-1 (the rat is awake from anesthesia), if the rat appears when the tail is lifted upside down The right upper limb bends to the front of th...

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Abstract

The invention belongs to the field of biomedicine and particularly relates to application of bumetanide in promoting neural functional reconstruction in the recovery period after ischemic stroke, particularly the application of bumetanide in neural functional reconstruction drugs for the recovery period after ischemic stroke. Studies discover that bumetanide is capable of promoting regeneration of axons in a rat with ischemic stroke, remodeling of axon synapsis and recovery of motor nerve functions, up-regulating NogoA expression level of the periphery of ischemic cortex, up-regulating BDNF (brain-derived neurotrophic factor) protein expression level of the periphery of the ischemic cortex, and promoting regeneration of axons by up-regulating the BDNF protein expression level of the periphery of the ischemic cortex and down-regulating the NogoA level.

Description

Technical field [0001] The invention belongs to the field of biomedicine, and particularly relates to the application of bumetanide in promoting the reconstruction of nerve function during the recovery period after ischemic stroke. Background technique [0002] Stroke is one of the main causes of clinical disability in adults. It has caused a great burden on patients, family members and the medical care system. Although a large number of studies have been conducted on stroke, there are currently effective treatment methods for it. It is still limited. Although acute thrombolytic therapy is effective, it is limited by the time window of 3-6 hours, so for most patients, neurological deficit is inevitable. Therefore, finding new targets and mechanisms of action and related drugs is a hot spot in the treatment of stroke. [0003] Many studies have shown that after cerebral infarction or other brain injury, the axons of cortical neurons on the injured side and the contralateral hemisph...

Claims

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Application Information

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IPC IPC(8): A61K31/196A61P25/00
CPCA61K31/196
Inventor 赵传胜程希穆晓鹏王慧彬于航赵岚青段思宁刘婷婷孙叶飞
Owner THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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