Preparation of enzymatic chlorambucil-polypeptide hydrogel and application in resisting cancer

A technology for catalyzing chlorambucil and hydrogels, applied in the field of polypeptide hydrogels, can solve the problem that the influence of polypeptide folding, self-assembly and function is rarely studied, and achieve the effect of improving activity

Active Publication Date: 2017-11-03
NANKAI UNIV
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Problems solved by technology

However, their impact on peptide folding, self-assembly, and function has been poorly studied

Method used

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  • Preparation of enzymatic chlorambucil-polypeptide hydrogel and application in resisting cancer
  • Preparation of enzymatic chlorambucil-polypeptide hydrogel and application in resisting cancer
  • Preparation of enzymatic chlorambucil-polypeptide hydrogel and application in resisting cancer

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Embodiment 1

[0018] (1) According to Document 1, the phosphorylated polypeptide CRB-G was synthesized by FMOC-solid-phase synthesis method D f D f p D Y (ALP, 4°C), its nuclear magnetic spectrum and mass spectrometry results are as follows:

[0019] 1 H NMR (400MHz, DMSO) δ8.32(d, J=7.5Hz, 1H), 8.22(d, J=8.1Hz, 1H), 7.97(d, J=15.2Hz, 2H), 7.29-7.16(m , 9H), 7.04(dd, J=27.5, 8.1Hz, 3H), 6.65(d, J=8.3Hz, 2H), 4.55(d, J=8.9Hz, 1H), 4.50-4.36(m, 2H) , 3.67(d, J=12.4Hz, 5H), 3.53(dd, J=19.7, 12.5Hz, 2H), 3.47-3.38(m, 1H), 3.03(d, J=11.3Hz, 2H), 2.97- 2.88(m, 2H), 2.84-2.63(m, 3H), 2.54(s, 1H), 2.46-2.35(m, 2H), 2.08(t, J=7.3Hz, 2H), 1.77-1.64(m, 2H).M+=897.27, obsvd.(M+H) + =898.2741, (M+Na) + =920.2544.

[0020] (2) Take 1 mg phosphorylated polypeptide CRB-G D f D f p D Y was placed in a 1.5 ml glass bottle, 990 microliters of PBS (pH=7.4) solution was added, the pH value was adjusted to 7.4 with sodium carbonate solution, 1 microliter of 1U / microliter alkaline phosphatase was add...

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Abstract

The invention relates to preparation of an enzymatic chlorambucil-polypeptide hydrogel and application in resisting cancer. The enzymatic chlorambucil-polypeptide hydrogel is prepared from folding phosphopeptides into alpha-helical configuration through catalytic specificity of alkaline phosphatase, and has the effect on remarkably improving the activity of an anti-tumor medicine. According to the application, the polypeptide sequence is CRB-G<D>F<D>F<D>Y and CRB-G<D>F<D>Fp<D>Y.

Description

technical field [0001] The invention relates to a polypeptide hydrogel formed under the catalysis of alkaline phosphatase and its application in improving the activity of antitumor drugs. Background technique [0002] Hydrogels formed by self-assembled polypeptides have the characteristics and advantages of good biocompatibility and high biological activity. Research in related fields has been a hot spot in the past 20 years. It has shown high application prospects in regenerative medicine, drug controlled release, analysis and detection, tissue engineering, etc. Polypeptide folding is crucial for proteins to form stable tertiary structures, obtain biological functions, and assemble into protein complexes. Peptide misfolding and protein misassembly lead to loss of protein function and are associated with disease. Inspired by spontaneous self-assembling systems, self-assembling peptides have been widely used in the construction of biomaterials. With the help of non-covalen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02A61K9/06A61K38/07A61P35/00
CPCA61K9/06A61K38/07C12P21/02
Inventor 杨志谋王玲梁春惠郑德彬石芳
Owner NANKAI UNIV
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