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Myocardial infarction biomarker miR-1283

A technology of mir-1283 and myocardial infarction, applied in the field of application of myocardial infarction biomarker miR-1283, miR-1283 and its target genes in the diagnosis and treatment of myocardial infarction

Inactive Publication Date: 2017-11-03
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the expression and possible role of miRNAs in acute myocardial infarction are less studied, previous studies have shown that miR-30a is associated with cardiac hypertrophy, miR-195 target genes regulate apoptosis, cell proliferation and cell cycle, they may Potential clinical value as a biomarker for the diagnosis of acute myocardial infarction, but these are far from enough, more candidate molecular markers are needed for accurate clinical diagnosis and treatment

Method used

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  • Myocardial infarction biomarker miR-1283
  • Myocardial infarction biomarker miR-1283
  • Myocardial infarction biomarker miR-1283

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Collection of samples and extraction of total RNA

[0048] The peripheral blood of patients with myocardial infarction and 6 cases of healthy controls were collected in the hospital from March 2015 to September 2016. Diagnostic criteria for acute myocardial infarction: developed according to the diagnostic criteria recommended by the third "Global Unified Definition of Myocardial Infarction" in 2012. An increase and / or decrease in the level of myocardial necrosis markers (mainly troponin) was detected, exceeding the 99th percentile value of the upper limit of the reference value at least once, and accompanied by at least one of the following ischemic symptoms.

[0049] 1) Symptoms of myocardial ischemia;

[0050] 2) New ischemic ECG changes;

[0051] 3) Pathological Q waves appear in the electrocardiogram;

[0052] 4) Imaging evidence shows new loss of myocardial activity or new local wall motion abnormality;

[0053] 5) Coronary angiography or autopsy fou...

Embodiment 2

[0055] Example 2 Sequencing, data analysis and electronic verification

[0056] Sequencing: miRNA and mRNA were sequenced using llumina Hiseq2500 / Miseq second-generation high-throughput sequencing technology, and data processing was completed by removing adapters, removing low-quality, and decontaminating processes to obtain final data. The miRNA and mRNA sequencing raw data were background-corrected by the transcriptome data analysis software, and then the t-test was performed to obtain the P value, and then the Fisher test was used to combine the P values ​​to screen the differentially expressed miRNA and mRNA. Use algorithms including miRanda, miRDB, miRWalk and Targetscan to predict the target genes of differentially expressed miRNAs, select ≥ 4 target genes predicted by the algorithms, and search for verified target genes of differentially expressed miRNAs in the miRWalk database.

[0057] Finally, miR-1283 and its target genes HDAC4, PHC2, THOC5, and IL17RA were selected...

Embodiment 3

[0058] Embodiment 3 Electronic Verification

[0059] Electronic verification: 3 sets of mRNA data sets (GSE48060, GSE34198 and GSE61145-GPL6884) and 2 sets of miRNA data sets (GSE61741, GSE31568) were screened from the GEO (Gene Expression Omnibus) database, and 3 sets of mRNA data sets (GSE48060, GSE34198 and GSE61145 -GPL6884) has a total of 13,680 genes. We calculated and merged the effect value by using the metaMA package and using the combined P-value method to obtain 612 (FDR1) were found. The results showed that the electronic verification results were consistent with the expression trend of the sequencing results.

[0060] The method for evaluating the efficacy of a single miRNA molecule or a diagnostic model is to establish a receiver operating characteristic (ROC) curve, and judge the ability of diagnosis by calculating the area under the curve (Area UnderCurve). The value of the area under the ROC curve is between 1.0 and 0.5. In the case of AUC>0.5, the closer the...

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Abstract

The invention relates to a myocardial infarction biomarker miR-1283, and concretely relates to the miR-1283 and application of target genes of the miR-1283 in treating myocardial infarction. The expression and the possible effect study of the miRNA in acute myocardial infarction are relatively few, and an existing biomarker is far from satisfying the clinic diagnosis requirement. On the basis of a high-throughput technology, the miR-1283 and the target genes HDAC4, PHC2, THOC5 and IL17RA of the miR-1283 are selected, and the confirmatory experiment shows that the miR-1283 and the target genes of the miR-1283 can be applied to clinical diagnosis and prevention detection of myocardial infarction; the myocardial infarction biomarker miR-1283 provided by the invention lays a foundation for research and development of related diagnostic reagents of myocardial infarction clinically.

Description

technical field [0001] The present invention relates to the field of disease diagnosis and treatment, in particular to miR-1283, a biomarker of myocardial infarction, and more specifically to the application of miR-1283 and its target gene in the diagnosis and treatment of myocardial infarction. Background technique [0002] Acute myocardial infarction (AMI) is the most serious cardiovascular disease, and early and accurate diagnosis can ensure the immediate initiation of reperfusion therapy and possibly reduce mortality. Some biomarkers such as cardiac troponin I have been applied to diagnose AMI, which can improve the current diagnosis and become a new method for acute myocardial infarction. However, the expression and possible role of miRNAs in acute myocardial infarction are less studied, previous studies have shown that miR-30a is associated with cardiac hypertrophy, miR-195 target genes regulate apoptosis, cell proliferation and cell cycle, they may The potential clin...

Claims

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Application Information

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IPC IPC(8): C12Q1/68A61K45/00A61P9/10
CPCA61K45/00C12Q1/6883C12Q2600/158C12Q2600/178
Inventor 孙锦云李曙光
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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