Method used for promoting rapid germination and seedling formation of paris polyphylla seeds

A seed and replant technology, applied in seed immunization, seed and rhizome treatment, germination equipment, etc., can solve the problems of troublesome seed treatment operation and long germination time, and achieve the effects of shortening germination time, increasing germination rate, and easy survival

Active Publication Date: 2017-11-07
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to overcome the shortcomings of cumbersome seed treatment operation and long germination time in the existing germination technology of chrysanthemum seeds, the present invention provides a method of combining sodium nitroprusside with intermittent submerged bioreactor to rapidly germinate chrysanthemum seeds method

Method used

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  • Method used for promoting rapid germination and seedling formation of paris polyphylla seeds

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Experimental program
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Embodiment 1

[0022] (1) Take the mature seeds of Aescinus chinensis picked in the same year, remove the glumes, put them into a breathable gauze bag, rub the red outer testa by hand, and then wash the Aescines aescini seeds with clean water Rinse 3 to 4 times to completely remove the testa residue. Then place it on a newspaper, dry it in the shade indoors for 3 to 5 days, and put it in a refrigerator at 4°C for later use.

[0023] (2) Rinse the new reactor with clean water, then fill the reactor with 1L of clean water, observe its airtightness and connect the reactor filled with clean water to the pump, and observe whether the filter tip flows backwards. Put the sealed reactor into an autoclave for sterilization at 121° C. for 20 minutes. After the reactor cools down, wrap it with newspaper and sterilize it again.

[0024] (3) Soak the dried Aescinus seeds in water for 24 hours to make the seeds fully imbibition and become round and plump. Then in the ultra-clean workbench, first wash th...

Embodiment 2

[0030] The germination experiment was carried out on the seeds of Chonglou dianthus picked in the same year. After removing the glume, the red outer testa was manually rubbed off by hand, and the outer testa was slowly sieved with a fine-mesh sieve, and then the Aescinus aesculus was washed with flowing water. Rinse the seeds 3-4 times with water to completely remove the outer testa residues. Then place it on a newspaper, dry it indoors in the shade for 3-5 days, and put it in a refrigerator at 4°C for later use.

[0031] Soak the dry Prunus edulis seeds in clean water for 24 hours, so that the seeds can fully imbibition and become round and plump. Then, sterilize the seeds in an ultra-clean workbench, put them into an artificial climate incubator for dark cultivation at 20±2°C, and observe after cultivating for 5 days. The dishes are washed away, and the thoroughly sterilized seeds are moved to the reactor for cultivation.

[0032] After sterilizing the surroundings of the ...

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Abstract

The invention provides a method used for promoting rapid germination and seedling formation of paris polyphylla seeds. The method comprises following steps: harvested paris polyphylla seeds are subjected to pretreatment; the leakproofness of a temporary immersion type bioreactor is detected, and the temporary immersion type bioreactor is subjected to sterilization; the paris polyphylla obtained via pretreatment are immersed in water overnight so as to realize full absorbing and swelling, and are subjected to sterilization at aseptic conditions, and detection is carried out so as to ensure complete sterilization is realized and no microbiological contamination is caused; the paris polyphylla seeds are delivered into the temporary immersion type bioreactor for culturing, wherein sterilized distilled water containing SNP is delivered into the temporary immersion type bioreactor, inoculation with the paris polyphylla seeds is carried out, seed temporary immersion frequency is controlled to be 3 to 5min / 6h, and culturing is carried out at a dark environment. According to the method, sodium nitroprusside is combined with the temporary immersion type bioreactor, so that rapid germination of paris polyphylla seeds is realized.

Description

technical field [0001] The invention relates to a method for rapid germination and seedling growth of pachyrhiza seeds by combining sodium nitroprusside (SNP) with an intermittent submerged bioreactor, and belongs to the technical field of rare medicinal plant propagation. Background technique [0002] Paris (Paris) is a perennial herb of the genus Paris in the family Liliaceae. The drug source of commonly used medicinal materials. Modern pharmacology shows that the rhizome of Chonglou has sedative and analgesic, antiasthmatic, hemostatic, antibacterial and anti-inflammatory effects, especially in recent years, it has been found to have a significant effect on anti-tumor. Therefore, driven by economic interests, the wild resources of Zhonglou are continuously plundered and excavated, and they are on the verge of extinction. Therefore, artificial breeding can not only protect the wild resources of Dianzhonglou, but also meet the growing market demand. [0003] The artifici...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01C1/02A01C1/08
CPCA01C1/00A01C1/02A01C1/08
Inventor 孟艳艳宋发军林爱华罗忠黄珍刘祖懿李海明李梅香
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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