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Antibacterial peptide and antibiotic combined antibacterial drug and use method thereof

A technology of antibacterial drugs and polypeptide antibiotics, which is applied in antibacterial drugs, pharmaceutical formulas, active ingredients of heterocyclic compounds, etc., can solve problems such as high medical expenses, bacterial aggravation, long hospital stay and death, and achieve good bactericidal effect, antibacterial Improve the effect and reduce the effect of side effects

Pending Publication Date: 2017-11-07
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The emergence of antibiotics has saved countless lives, but the problem of bacterial resistance to antibiotic products has also increased year by year. The speed of new drug research and development is far behind the speed of bacterial resistance. longer hospital stays and more deaths

Method used

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  • Antibacterial peptide and antibiotic combined antibacterial drug and use method thereof
  • Antibacterial peptide and antibiotic combined antibacterial drug and use method thereof
  • Antibacterial peptide and antibiotic combined antibacterial drug and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 The synergistic antibacterial effect of DP7 combined with various antibiotics on various clinically isolated drug-resistant bacteria and the synergistic antibacterial effect comparison with CLS001:

[0043] The antimicrobial peptide DP7 (amino acid sequence VQWRIRVAVIRK-NH2, with amidation at the carbon terminus) was combined with the antibiotic drugs vancomycin (VAN), gentamicin (GEN), azithromycin (AZT) and amoxicillin (AMO) with checkerboard Antibacterial experiments were carried out by drug combination, and selected clinically isolated drug-resistant Staphylococcus aureus strains (SAU series), clinically isolated drug-resistant Escherichia coli strains (ECO series), and clinically isolated drug-resistant Escherichia coli strains (ECO series) obtained from Chongqing Southwest Hospital and Sichuan Provincial Center for Disease Control and Prevention. Three isolated drug-resistant Pseudomonas aeruginosa strains (PAER series) and three clinically isolated drug-...

Embodiment 2

[0067] Example 2 The expansion experiment of DP7 combined with vancomycin and azithromycin for antibacterial

[0068] When the antimicrobial peptide DP7 is used in combination with vancomycin and azithromycin, it has a better synergistic antibacterial effect on the clinically isolated drug-resistant strains of Staphylococcus aureus and Pseudomonas aeruginosa, so we expand the combination of DP7 with vancomycin and azithromycin The number of strains of Staphylococcus aureus clinically isolated drug-resistant bacteria and Pseudomonas aeruginosa clinically isolated drug-resistant bacteria in the joint antibacterial experiment is used to further study the synergistic antibacterial effect.

[0069] Real-time quantitative PCR (qPCR) was used to determine the accuracy of clinical isolation of drug-resistant bacteria. Use DNeasyBlood&Tissue Kit (Qiagen) to extract the total DNA of bacteria, and detect the DNA concentration with a Thermo Scientific NanoDrop2000 spectrophotometer. The c...

Embodiment 3

[0074] Example 3 The relationship between the synergistic effect of DP7 and azithromycin and the azithromycin resistance gene

[0075] Based on the synergistic antibacterial effect of DP7 combined with azithromycin or vancomycin and the resistance of each strain to azithromycin or vancomycin, we used real-time quantitative PCR (qPCR) to clinically isolate drug-resistant Staphylococcus aureus and The clinical isolation of Pseudomonas aeruginosa drug-resistant bacteria has carried out the identification and analysis of common vancomycin resistance genes and azithromycin resistance genes. The drug resistance genes involved in this example are vancomycin resistance genes (vanB, vanC) and azithromycin resistance genes. Drug resistance genes (ermA, ermB, ermC, mefA, msrA).

[0076] The results are shown in Table 4 and Table 5. We concluded from the qPCR gene identification results that the isolated Staphylococcus aureus drug-resistant bacteria and Pseudomonas aeruginosa clinically i...

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Abstract

The invention belongs to the field of biomedicine, and mainly relates to an antibacterial peptide and antibiotic combined antibacterial drug and a use method thereof. The invention aims to provide a novel effective choice for the anti-infective therapy, and provides an antibacterial drug. The antibacterial drug comprises main active components: antibacterial peptide and antibiotic. The drug can effectively improve the antibacterial effect. The using amount of drugs, in particular antibiotics, is reduced; the side effect and toxic effect are reduced therefore; the drug resistance of strains is effectively reduced; the killing effect on drug resistant strains is also very good, and a novel effective choice is provided for anti-infective therapy.

Description

technical field [0001] The invention belongs to the field of biomedicine, and mainly relates to an antibacterial drug combined with an antimicrobial peptide and an antibiotic and a use method thereof. Background technique [0002] Antibiotics are medicines used to prevent and treat bacterial infections. The emergence of antibiotics has saved countless lives, but the problem of bacterial resistance to antibiotic products is also increasing year by year. The speed of new drug research and development is far behind the speed of bacterial resistance. Longer hospital stays and more deaths. Antibiotic resistance is the development of resistance to antibiotics in common bacteria that cause infections. It occurs when bacteria respond to the use of such drugs and change, usually due to resistance genes. [0003] In the European Union alone, drug-resistant bacteria were responsible for an estimated 25,000 deaths in 2014 alone, costing more than $1.5 billion in health care spending a...

Claims

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Application Information

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IPC IPC(8): A61K45/06A61K38/10A61K38/14A61K31/7052A61K31/43A61P31/04
Inventor 杨莉
Owner SICHUAN UNIV
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