Microfluidic chip for detecting tumor markers based on uniformly distributed microspheres, and use method thereof

Abstract

The invention relates to a microfluidic chip for detecting tumor markers based on uniformly distributed microspheres, and a use method thereof. The microfluidic chip comprises a sample inlet, a sample outlet and a plurality of micro-column arrays parallel to a liquid flowing direction, wherein the gaps between the micro-column arrays are main flowing channels, and the narrowest gap between the adjacent micro-columns in the micro-column arrays is slightly smaller than the diameter of the microsphere. According to the present invention, based on the principle of fluid diffusion, the structure is simple, the complex flow resistance theoretical calculation is not required, the microspheres can be uniformly arranged between the gaps of the micro-columns, and the interference of the fluorescence signals can be avoided with the discretely distributed microspheres; the microspheres can be combined with different antibodies according to the requirements so as to detect different markers; and the sample injection time can be shortened through the used incubation method, the sample injection and the washing are integrated so as to simplify the experiment operation, and the microfluidic chip can be used for the biochemical detection using the microspheres as the carrier, and has the good application prospects.

Description

technical field [0001] The invention belongs to the field of biosensors, in particular to a microfluidic chip for detecting tumor markers based on uniformly distributed microspheres and a method for using the same. Background technique [0002] In May 2015, the British "Daily Mail" reported: "The death rate caused by cancer is increasing year by year, and it is currently the second largest killer after cardiovascular disease." Statistics show that 5 years after early lung cancer resection The survival rate is 60-90%, while the 5-year survival rate of patients with low early diagnosis rate of lung cancer in my country is only 10%, and 70-90% of cancer patients die of tumor metastasis and recurrence within 5 years. Before the tumor metastasizes, the expression of some markers in the body has changed. If these markers can be detected accurately and effectively, early screening of tumors can be realized, and treatment measures can be taken in time, which can effectively improve t...

AI Technical Summary

Problems solved by technology

The method of driving or trapping microspheres with special properties by using magnetic or electric fields often requires equipment to generate external fields, which complicates the experimental steps

However, in the method of hydrodynamic interception, if the structure is not properly designed, the microspheres will often accumulate at a certain position in the channel, hindering the flow of liquid.

Moreover, when observing, the aggregated microspheres in the channel will overlap or cover, and the halo of each microsphere under the fluorescence will have a serious impact on the statistics of its fluorescence intensity, which is not conducive to accurate analysis

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