A method of in vitro regeneration using the leaves of Hexa officinalis

An in vitro regeneration and hexagram technology, applied in the field of in vitro regeneration using hexagram leaves, can solve the problems of difficult seedling production, low multiplication coefficient, low reproduction coefficient, etc., and prevent callus formation , increase the multiplication coefficient, the effect of increasing the multiplication coefficient

Active Publication Date: 2020-02-07
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this breeding method has a low reproduction coefficient and is time-consuming and labor-intensive
[0005] At present, there are few researches on the tissue culture of Helicia daflora at home and abroad, and most of the aseptic seedlings are obtained by inducing the growth point of terminal buds or axillary buds directly, and the multiplication is realized by means of sterile short branch cuttings. The coefficient is low, it is difficult to meet the needs of seedling production

Method used

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  • A method of in vitro regeneration using the leaves of Hexa officinalis
  • A method of in vitro regeneration using the leaves of Hexa officinalis
  • A method of in vitro regeneration using the leaves of Hexa officinalis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 A method for in vitro regeneration of leaves of the species "MARDI GRAS", the cultivation steps are as follows:

[0047] 1) Selection of explants: The explants are from the leaves of the "MARDI GRAS" variety planted in the nursery of Fengpu District, Shanghai Academy of Agricultural Sciences.

[0048] 2) Disinfection of explants: take the leaves as explants, clean them with a soft brush dipped in detergent, rinse them with running water for 1-2 hours, and disinfect them with 70% alcohol for 30 seconds on an ultra-clean bench , 0.1% HgCl 2 Disinfect for 10 minutes, rinse with sterile water for 5 times, and set aside.

[0049] 3) Adventitious bud induction: cut off the leaf tips and both sides of the leaves after disinfection, and cut them into 0.5×0.5cm leaf squares, see figure 1 , inoculate the leaves back down in adventitious bud induction medium, and culture in the dark at 25°C for 30 days to induce adventitious buds, see figure 2 , each leaf block can ...

Embodiment 2

[0057] Embodiment 2 A method for the in vitro regeneration of leaves of the species "TWIST OF LIME" of Hexa japonica, the cultivation steps are as follows:

[0058] 1) Selection of explants: The explants come from the leaves of the "TWIST OF LIME" variety plant of the "Twist of Lime" plant planted in the nursery of Fengpu Park, Shanghai Academy of Agricultural Sciences.

[0059] 2) Disinfection of explants: Take the leaves as explants, clean them with a soft brush dipped in detergent, rinse them under running water for 1 to 2 hours, and disinfect them with 70% alcohol for 60 seconds on an ultra-clean bench , 0.2% HgCl 2 Disinfect for 6 minutes, rinse with sterile water 5 times at the end, and set aside.

[0060] 3) Adventitious bud induction: cut off the leaf tip and both sides of the sterilized leaves, cut them into 0.5×0.5 cm leaf squares, inoculate the leaves back down in the adventitious bud induction medium, and culture them in the dark at 25°C for 40 Adventitious buds ...

Embodiment 3～4 and comparative example

[0077] In Examples 3 and 4, except that the formulations of the rooting medium are different, all other cultivation methods refer to Example 2 of the present invention, and comparative examples 1-2 are set up, and the formulations of the medium and the comparison results are shown in Table 3.

[0078] table 3

[0079]

[0080] As can be seen from Table 3, the auxin concentration is an important factor affecting the rooting of A. grandiflora, without adding any auxin medium (comparative example 1) or when the auxin concentration is low (comparative example 2), the The rooting rate is low and it is difficult to meet the production demand.

[0081] In the present invention, two kinds of auxins IBA and NAA are used together, which can effectively improve the rooting rate of A. daflora. When the concentration of the auxin continues to increase, the rooting rate does not increase significantly. Therefore, the proper concentration ratio of IBA and NAA can maximize the rooting rat...

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Abstract

The invention discloses a method for in vitro regeneration by use of leaves of abelia grandiflora. The leaves of abelia grandiflora are taken as an explant, rooting seedlings are obtained after adventitious bud induction, cluster bud proliferation and rooting culture, and saplings of abelia grandiflora are obtained through seedling hardening and domestication. 6-BA is added to an inducing medium, the leaf explants can be directly induced to come into adventitious buds, 6-BA and NAA are added to a proliferation medium, adventitious buds can grow into cluster buds, and the proliferation purpose is achieved; IBA and NAA are added to a rooting medium, so that basal stems form adventitious roots. Tissue culture of abelia grandiflora is conducted with the method, a direct organ regeneration technology of abelia grandiflora is established, the reproductive time of the saplings can be shortened, the production efficiency of the seedlings is improved, and the regeneration method can be used for an acceptor system for genetic transformation of abelia grandiflora.

Description

technical field [0001] The invention belongs to the field of induced regeneration of explants, and in particular relates to a method for in vitro regeneration by utilizing the leaves of Hexa officinalis. Background technique [0002] Abelia grandiflora (Abelia grandiflora) is a plant of the genus Abelia in the family Caprifoliaceae. It is an earlier horticultural species of Abelia. It is a dwarf variety of Abelia. It has been introduced in China, but the number is very large limited. It is a hybrid of glutinous rice sticks (A. chinensis) native to China and A. uniflora (A. uniflora), and has been bred abroad to form several more distinctive horticultural varieties. [0003] It grows fast and has a very beautiful plant shape. The branches are supple and drooping, and the tree is dancing. It is very suitable whether it is used as a matching plant in the garden or as a group plant for hedges and flower paths. The flowering period is long, and every year from early summer to m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李水根关媛苏振洪朱建军李秀芬李记开范晓芬
Owner SHANGHAI ACAD OF AGRI SCI
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