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Enzymolysis extraction method for extracting polysaccharide from tremella fuciformis

An extraction method and enzymatic hydrolysis technology, which are applied in the field of enzymatic hydrolysis extraction, can solve the problems of destroying the active structure of polysaccharides, loss of extraction rate of polysaccharides, and difficulty in extraction, and achieve the effects of improving enzymatic hydrolysis efficiency, avoiding polysaccharide loss and increasing added value.

Inactive Publication Date: 2017-11-21
姚莉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the common extraction method of Tremella polysaccharide is hot water extraction, but this traditional extraction method has problems such as low extraction rate, time-consuming, and waste of resources.
Tremella polysaccharides are divided into extracellular polysaccharides and cell wall polysaccharides. It is difficult to extract the cell wall polysaccharides completely with water. Even with dilute acid or dilute alkali extraction methods, some polysaccharides are prone to hydrolysis, which destroys the active structure of polysaccharides, resulting in the loss and extraction of polysaccharides. rate reduction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] An enzymatic extraction method for extracting polysaccharides from white fungus, comprising an enzymatic hydrolysis step and an extraction step, the enzymatic hydrolysis step refers to mixing white fungus powder and deionized water in a ratio of 1:60, and adjusting the pH to After 6, add compound enzyme, after 1 hour of enzymatic hydrolysis, quickly heat up to 90°C to inactivate the enzyme, and obtain the enzymatic hydrolysis solution; the extraction step refers to placing the enzymatic hydrolysis solution in a circulating ultrasonic extraction machine for extraction, and the extraction time is 0.5h , after filtration, obtain filtrate and filter residue;

[0014] The compound enzyme is formed by mixing protease, cellulase and pectinase in a mass ratio of 0.2:1:3.

Embodiment 2

[0016] An enzymatic extraction method for extracting polysaccharides from white fungus, comprising an enzymatic hydrolysis step and an extraction step, the enzymatic hydrolysis step refers to mixing white fungus powder and deionized water at a ratio of 1:70, and adjusting the pH to After 6, add compound enzyme, after enzymolysis for 1.5h, quickly heat up to 90°C to inactivate the enzyme, and obtain the enzymolysis solution; the extraction step refers to placing the enzymolysis solution in a circulating ultrasonic extraction machine for extraction, and the extraction time is 0.7 h, after filtering, obtain filtrate and filter residue;

[0017] The compound enzyme is formed by mixing protease, cellulase and pectinase in a mass ratio of 0.3:1.1:4.

Embodiment 3

[0019] An enzymatic extraction method for extracting polysaccharides from white fungus, comprising an enzymatic hydrolysis step and an extraction step, the enzymatic hydrolysis step refers to mixing white fungus powder and deionized water in a ratio of 1:80, and adjusting the pH to After 7, add compound enzyme, after 2 hours of enzymatic hydrolysis, quickly heat up to 90°C to inactivate the enzyme, and obtain the enzymatic hydrolysis solution; the extraction step refers to placing the enzymatic hydrolysis solution in a circulating ultrasonic extraction machine for extraction, and the extraction time is 1 hour. After filtering, a filtrate and a filter residue are obtained;

[0020] The compound enzyme is formed by mixing protease, cellulase and pectinase in a mass ratio of 0.5:1.2:5.

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Abstract

The invention discloses an enzymolysis extraction method for extracting polysaccharides from white fungus, which comprises an enzymolysis step and an extraction step. The enzymolysis step refers to carrying out tremella powder and deionized water at a ratio of 1:60-80 Mix and adjust the pH to 6-7, then add the complex enzyme, and after 1-2 hours of enzymolysis, quickly raise the temperature to 90°C to inactivate the enzyme to obtain the enzymatic hydrolysis solution; the extraction step refers to placing the enzymatic hydrolysis solution in a circular ultrasonic extraction Extract in the machine, the extraction time is 0.5~1h, after filtering, obtain the filtrate and filter residue; the compound enzyme is composed of protease, cellulase and pectinase in a mass ratio of 0.2~0.5:1~1.2:3~5 Mixed proportions. The invention effectively avoids the loss of the polysaccharide in the white fungus, and at the same time greatly improves the enzymolysis effect and efficiency.

Description

technical field [0001] The invention belongs to the technical field of edible fungus deep processing, in particular to an enzymatic extraction method for extracting polysaccharides from white fungus. Background technique [0002] Tremella fuciformls (Tremella fuciformls) is a kind of edible and medicinal mushroom with high economic value and rich nutrition. It has the effects of nourishing body fluid, moistening lung and nourishing stomach. [0003] Tremella polysaccharide is the main active ingredient of Tremella, which has the functions of improving the body's immunity, lowering blood sugar, lowering blood fat, anti-aging, anti-ulcer, anti-thrombosis, and anti-mutation. At present, the common extraction method of tremella polysaccharide is hot water extraction, but this traditional extraction method has problems such as low extraction rate, time-consuming, and waste of resources. Tremella polysaccharides are divided into extracellular polysaccharides and cell wall polysac...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 姚莉
Owner 姚莉